S. cerevisiae is an excellent reference organism for biomedical research in investigating fundamental cellular processes. In spite of their small size, the cellular morphology and physiology of yeast cells can be successfully studied thanks to microscopy techniques such as indirect Immunofluorescence and fluorescent live cell imaging. Indirect immunofluorescent protocols for specific and homogenous staining are typically easily applied, but at the cost of nonviable cells and time-consuming preparation of slides (procedure prone to problems with fixation and permeability). On the other hand, setting up live-cell, time-lapse microscopy requires expensive equipment and labeling with organelle-specific fluorescent dyes expression can be critically dependent on the yeast growth phase. Nanolive’s technology wants to bypass these limits, allowing for a 3D live-cell imaging without any need for chemical markers or genetic manipulation.
3D Cell Explorer allows for:
- Real-time acquisitions of the budding phenomena (3 hours time-lapse, highest acquisition rate: 3D reconstruction in less than 2 seconds)
- Studying differences in bud and mother yeast (different composition, volume, RI, vacuole dimension etc.)
- Direct measures of cytoplasm/vacuole volume during the growing/starvation/aging phenomena
- Better morphological view of the yeast (localization of yeast and buds in three dimensions)
Budding Yeast: S. cerevisiae
S. cerevisiae strand (FRCs) was grown complete in SCD (Synthetic Complete Dextrose) medium in 35mm glass bottom culture dishes (ibidi µ-Dish). The time-lapse imaging experiment was conducted at RT for 3 hours, capturing images every 30 seconds. The different components are digitally stained based on their specific Refractive Index (RI): membrane (purple), cythosol (yellow), vacuole (black).
Learn more about the 3D Cell Explorer
The 3D Cell Explorer acts now as a cell GPS. Follow your cells and monitor their speed in real-time!