The landscape of live cell imaging today is composed by two major type of techniques. From one side there is traditional fluorescence microscopy which includes different techniques among which some super-resolution microscopy (Nobel Prize for Chemistry, 2014). These techniques definitely allow to make new discoveries by pushing the resolution further than ever before but they face few important limitations: They requires to impair the cells, by fixation or by genetic modification with the risk of results falsification and they damage the cells by applying very high intensity laser sources which are phototoxic for the cells and bleach the sample already after short imaging intervals. This fundamentally limits scientists to perform research on living cells: they are cell friendly limited. On the other side, there are marker-free technologies which are potentially very cell friendly like holography or phase contrast. Trouble is that they lack of specificity, quantitative nature and they hardly allow for functional imaging schemes. This fundamentally limits scientists to employ these techniques in their research: they are discovery limited.
Now, if you look at this landscape, it leaves you with a big void in the middle: a whole uncharted world for live cell imaging. Nanolive wants to reveal this world and it wants to do it thanks to its revolutionary microscope: the 3D Cell Explorer.
The 3D Cell Explorer has the potential to be the ultimate live cell imaging tool:
- Marker-free: so completely non-invasive to your cells
- It allows for unlimited live cell imaging in physiological conditions (up to several days).
- No bleaching
- No phototoxicity due to the extremely low power of illumination
- It delivers a full 3D reconstruction every second
- It delivers quantitative results by measuring the cell’s inherent physical properties: its refractive index (RI)
- The 3D Cell Explorer is compatible with traditional live cell imaging consumables and flow chambers
- what is necessary to achieve infinite 4D live cell imaging
- how to create authentic physiological conditions on the stage of your microscope: top-stage incubator
- how to run a infinite time-lapse experiment with the 3D Cell Explorer
- how to obtain authentic label-free, quantitative 3D cell images
This will be followed by several examples of applications and exciting experiments performed with the 3D Cell Explorer