TechnologyInside the 3D Cell Explorer
Tomography of a Neuron
Living cell tomography
The Cell Explorer’s technology is unique worldwide and is based on a fundamental patent (US 8,937,722 B2 & EU WO 2011/121523).
It allows determining how light propagates through the cell, called the refractive index (RI). By this means, one can measure the cell’s physical properties. The result is quantitative cell tomography, in vitro without any invasion or sample preparation.
Learn more about our technology in our recent publication:
Cotte Y. et al., Marker-free phase nanoscopy, Nature Photonics, 2013.
Self-adjusting & holographic tomography
The combination of holography and rotational scanning makes Nanolive a revolutionary technology.
Holography offers a unique means to measure cells in their native environment: Label-free, non-invasive, manipulation-free, and interference-free. Rotational scanning allows 3D reconstructions, noise robustness, and a resolution far beyond the accepted limit for light.
To achieve optimal results for every measurement, the optics of our technology are completely self-adjusting. It guarantees the best alignment conditions – whatever the environment is.
Standard traditional fluorescent microscopy
Traditional microscopy is limited by the need of chemical fluorescent molecules reporters in order to visualize the different cell components. The user has to decide prior to experiment which stains he may need to prepare the sample with. The typical time for this procedure is 1-72 hours and just a few stain are possible on the same sample.
The Nanolive technology
The Nanolive technology provides a measurement of the RI distribution within the cell. Therefore, the user can decide after the experiment which cell parts he/she wishes to ‘digitally stain’ in a limitless amount of colours – saving a lot of time and money on reagents.
Allowing to measure with nanometric details
We believe Nanolive’s technology could create a new paradigm in optical microscopy of living cells.
The Nanolive technology enables:
- Non-invasively measuring the world of living cells with nanometric details
- RI distribution within the cell (3D) and in time lapse (4D)
- Comprehensive results in real time
Since the cells are not manipulated in any way to introduce a label, it gives the user the possibility to measure the response of authentic cell’s physiological activity. The measurement of cellular processes with real-time kinetics enables multi-parameter analysis at single cell and sub-cellular scale.