Endothelial cells, as mentioned in our previous blogpost (https://nanolive.ch/failed-mitotic-exit-in-human-umbilical-vein-endothelial-cells/), constitute the inner layer of blood and lymphatic vessels. They also play a role in substance and cell turnover between bloodstream and adjacent tissues.
Human Umbilical Vein Endothelial Cells (HUVECs) are a well-established model system used to study endothelial cells regulation and behaviour. PromoCell GmbH kindly provided us with a high quality single donor, cryopreserved HUVEC culture. A sample of HUVECs incubated with endothelial cell growth medium and fibronectin was observed under Nanolive’s 3D Cell Explorer (1 image every 15 seconds for 6 hours and 30 minutes).
HUVEC culture with fibronectin
Fibronectin is a glycoprotein of the extracellular matrix that facilitates attachment and cytoplasmic spreading of in vitro cells. Addition of fibronectin has been proved to increase survival and adherence of HUVEC cells in culture , .
In order to maintain integrity of the endothelium, endothelial cells show a great number of cell-cell interactions, mainly tight-junctions and adherens junctions, .
The maintained cell-cell interactions observed in the sample may respond to HUVECs’ natural role regarding the formation of layers in veins (Figure 1). Tight junctions and adherent junctions control vascular permeability in endothelium–.
During angiogenesis, cell-matrix interactions are crucial (see Figure 1). Such structures are involved in migration, invasion and survival of cells throughout the angiogenic process .
We can also observe filopodia and lamellipodia with great details (Figure 1) thanks to Nanolive’s imaging capacity to catch membrane deformations. Those structures are essential for migration, cell-cell interactions or environment sensing, amongst others. While filopodia appear as long, thin protrusion-like structures that emerge from the cellular membrane, lamellipodia are broad, sheet-shaped structures containing thin and short interconnected actin filaments.
Cell-matrix interactions are vital in cancer-related angiogenesis, –, and HUVEC cultures are optimal for research in this topic–.
Figure 1. Cell-cell and cell-matrix interactions in HUVEC culture. Image extracted from long-term live cell imaging footage with the 3D Cell Explorer.
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