Nanolive is pleased to share a new publication in Nature Scientific Reports from our users in the Justus-Liebig University of Giessen .
Besnoitia besnoiti is an emerging parasite in Europe, responsible for bovine besnoitiosis, an illness causing a significant economic impact on cattle industry.
Similarly to T. gondii, already featured here, B. besnoiti also needs to scavenge cholesterol from its host cell in order to replicate. However, the mechanism used by B. besnoiti to obtain cholesterol is still controversial.
On this work, Silva and colleagues study the model of cholesterol salvage by analysing B. besnoiti infection of primary bovine endothelial cells in vivo.
Their results demonstrate that B. besnoiti can obtain cholesterol both via endogenous than exogenous uptake. This finding reinforces the theory that the successful B. besnoiti infections in primary bovine endothelial host cells rely on both the parasite and the cell type.
The 3D Cell Explorer- fluo was used to confirm the presence of abundant lipid droplet-like structures in B. besnoiti-infected cells. Images were later analyzed using STEVE software (Figure below).
The full publication is available here!
Figure 1. Live cell holographic tomography-based illustration of lipid droplets in non-infected and B. besnoiti- infected BUVEC.
 Silva, L. M. R. et al. Besnoitia besnoiti infection alters both endogenous cholesterol de novo synthesis and exogenous LDL uptake in host endothelial cells. Sci. Rep. 9, 6650 (2019).
Read our latest news
Mesenchymal stem cells (MSCs) hold great promise for regenerative medicines such as cell therapy and tissue engineering (1,2). These approaches require cells to be grown in culture and differentiated into specific cell types such as osteoblasts (bone cells),...
Stem cells were isolated from the umbilical cord matrix and grown in culture for 14 days. At this stage most cells in the population are at the mature megakaryocyte stage and can start producing platelets in internal membranes present in their cytoplasm. In this...
Feature Client: Michal Cifra from the Institute of Photonics and Electronics Academy of Sciences (Czech Republic)
Dr. Michal Cifra is the young, dynamic team leader of the Bioelectrodynamics team at the Institute of Photonics and Electronics Academy of Sciences (Czech Republic). His lab has recently purchased Nanolive’s 3D Cell Explorer-fluo microscope and published his first...
3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence