Nanolive’s newest microscope, the 3D Cell Explorer-fluo answers to the scientific need to combine the benefits from non-invasive cell tomography with a well-established and recognized method: multi-channel fluorescence microscopy. This opens completely new frontiers for research. Fluorescence microscopy offers the advantage to selectively visualize specific cell compartments by staining them with various fluorescent probes, while novel holographic tomography gives access to the whole 3D RI distribution within the cell. This combination allows to measure, for the first time, specific cell compartments in a totally non-invasive fashion, for an unlimited amount of time and in 3D. In addition, parallel time-lapse measurements of live cells with the two technologies can be used to investigate the 4D intracellular dynamics of individual organelles. It is therefore an unrivalled solution that opens the door to new dimensions for biological investigations. Researchers can now have access to two powerful imaging modalities at the same time; they can compare and correlate resulting data and add new information to their experiments.