Mitochondria play an essential role in neurone function. They provide the energy and metabolites needed to: (i) form and maintain the synapses (neural junctions) that exist between cells, (ii) remove or repair old/damaged components, and (iii) deliver new materials, most of which are encoded by nuclear genes1.
The long and complex morphology of a neurone, combined with the high demand for energy at the periphery of the cell, makes the trafficking of mitochondria essential2. At any given moment, 10 to 40% of mitochondria are estimated to be in motion within the cell with half moving away from the cell body and half moving towards the cell body1.
In this video, neuronal mitochondria were stained using a fluorescent dye and then imaged for 10 mins with the 3D Cell Explorer-fluo at an acquisition frequency of 1 image every 10 secs. Mitochondria can be seen being trafficked along axons, dendrites and within the cell body. They are also present in dendritic growth cones (actin-supported extensions of developing neurites).
Sample courtesy of Vanessa Morais and Rita Soares, iMM institute Lisbon.
- Schwarz, T. L. Mitochondrial trafficking in neurons. Cold Spring Harb. Perspect. Med. 3, 1–16 (2013).
- Mandal, A. & Drerup, C. M. Axonal transport and mitochondrial function in neurons. Front. Cell. Neurosci. 13, 1–11 (2019).
Read our latest news
Mesenchymal stem cells (MSCs) hold great promise for regenerative medicines such as cell therapy and tissue engineering (1,2). These approaches require cells to be grown in culture and differentiated into specific cell types such as osteoblasts (bone cells),...
Stem cells were isolated from the umbilical cord matrix and grown in culture for 14 days. At this stage most cells in the population are at the mature megakaryocyte stage and can start producing platelets in internal membranes present in their cytoplasm. In this...
Feature Client: Michal Cifra from the Institute of Photonics and Electronics Academy of Sciences (Czech Republic)
Dr. Michal Cifra is the young, dynamic team leader of the Bioelectrodynamics team at the Institute of Photonics and Electronics Academy of Sciences (Czech Republic). His lab has recently purchased Nanolive’s 3D Cell Explorer-fluo microscope and published his first...
3D CELL EXPLORER
Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging
3D CELL EXPLORER-fluo
Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence