Video caption: Label-free live cell imaging of a Preadipocyte cell where healthy mitochondria (long filaments) can be appreciated
Nanolive is happy to announce that we will participate with a talk and a booth in the “Targeting Mitochondria Congress” from 27th to 29th of October in Berlin.
The talk will take be hold on Monday October 28th at 3:40pm in the main lecture hall.
We are looking forward to meeting you there!
Non-invasive live cell imaging overcomes phototoxicity problem while imaging cellular metabolism processes
A major problem with current imaging techniques is phototoxicity that leads to the observation of perturbed dynamics. Consequently, the mitigation of phototoxicity leads to poor time resolution of time lapse approaches. This is particularly true for small organelles like mitochondria or lipid droplets that are extremely sensitive to photo-induced oxidation. Last but not least, the use of chemical or genetically-encoded fluorescent markers perturbs the targeted biological processes.
However, the 3D Cell Explorer overcomes this problematic as it injects in the sample ~100 times less energy (~0.2 nW/µm2) than light sheet microscopes that are the reference in the matter. With a resolution below 200 nm, it enables high resolution and high-frequency imaging even with sensitive material, giving access to organelle dynamics that were previously out of reach.
Read more on our dedicated mitochondria page here.