Top: 2D slice from the RI map of a mouse fibroblast and 3D reconstruction of the whole stack. Bottom: zoom into the internal compartments: a. nucleus + nuclear membrane, b. lipid droplets, c. lysosomes, d. plasma membrane, e. nucleoli, f. mitochondria.
The next symposium is around the corner: Nanolive will be holding a talk at the LS2 ANNUAL MEETING 2018 in Lausanne on Tuesday 13th of February at 14:00 at the AMPHIPÔLE / AMPHIMAX in Lecture Hall 351 where we will introduce our technology and its implications for live cell research.
The 3D Cell Explorer, a label-free nanoscopic solution to monitor host-pathogen interactions in three dimensions.
Luca Clario, Nanolive SA, Switzerland
In the era of live cell imaging, the need for higher lateral and axial resolution at subcellular level coupled with high temporal frequency becomes vital to follow complex and dynamic biological processes in 3D.
Nanolive’s 3D Cell Explorer – the first microscope of this kind – allows for the creation of high-content 3D images of living cells with very high spatio-temporal resolution (x, y:180nm; z:400nm; t:1.7sec) and minimal invasiveness.
Among its many different applications, this innovative imaging technique allows for new intriguing discoveries in the field of intracellular infection. With the 3D Cell Explorer, the progression of the pathogen infection inside unlabeled living cells can be monitored in real-time for long term (up to weeks) at a frequency of one 3D acquisition every 1.7s and a resolution of 200nm. Moreover, thanks to the quantitative nature of the refractive index measurements, the system enables for quantitative data analysis for infected cells (i.e. dry mass distribution and direct volume estimation).
Mef cell infected by Toxoplasma gondii.
We sincerely hope to see you at the LS2 ANNUAL MEETING.