On this page you will have the possibility to explore the cell culture with high confluency as we see it: in 3D and stain free! Enjoy and keep tuned: the list of results grows every week…
Nanolive’s technology allows for:
• Cellular multi-layer construction visualization
• Contact inhibition studies
In cell culture, confluence is the term commonly used as an estimate of the number of adherent cells in a culture dish or a flask, referring to the proportion of the surface which is covered by cells. For example, 100% confluency means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer. Many cell lines exhibit differences in growth rate or gene expression depending on the degree of confluence. Cells are typically passaged before becoming fully confluent in order to maintain their proliferative phenotype. Some cell types, not limited by contact inhibition, such as immortalized cells, may continue to divide and form layers on top of the parent cells. To achieve optimal and consistent results, experiments are usually performed using cells at a particular confluence, depending on the cell type.
The 3D Cell Explorer allows the acquisition of 3D images of your cell cultures in real-time and in a non-invasive way.
Lung cancer cells 100% confluency
Human lung cancer cells were grown to 100% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100).