Live FUCCI mESC were imaged for over 48 hours with Nanolive’s 3D Cell Explorer-fluo. A holotomographic image was taken every 14 seconds while a double channel (green and red) epifluorescence image was only taken every 30 holotomographic frames (7:30 minutes). The difference in acquisition rate was performed to reduce fluorescence related phototoxicity.
*Please note that the latest fluorescence image is displayed over the following 30 holotomographic images until a new one is acquired, for visualization purpose. The mismatch between the two signals is due to this image processing choice.
About the 3D Cell Explorer-fluo
Multimodal complete solution for 3D live cell exploration
Combine 3D refractive index analysis with a fully integrated 3 channel fluorescence module to image your live cells as they are and as long as you want. Put chemical information into structural context for new biological insights.
Transform 2D fluorescence into 3D cell tomography: Identify cell organelles through fluorescence and monitor non-invasively their structures and dynamics in 3D and marker-free. Explore fluorescence (3 channels) and Digital Stains (8 channels) simultaneously.
Extended live cell imaging
Image your live cells as long as you need. Limit cell damages caused by fluorescent markers, bleaching and phototoxicity.