Call to all microscopists!
Nanolive has had a number of scientific discussions recently on whether fluorescence based live cell imaging observations on cell/organelle dynamics and morphology can lead to incorrect conclusions if phototoxicity is not controlled for.
Visit our dedicated page on phototoxicity here: https://nanolive.ch/technology/overcoming-phototoxicity/.
As such we are proposing an open challenge to all live cell researchers using fluorescence based microscopy techniques.
Here is the null hypothesis: fluorescence based imaging can successfully be used to image living cells for over 48 hours with zero observable negative impact.
To prove this, email us with subject: “The fluorescence challenge” and any fluorescence imaging video of live cells lasting longer than 48 hours that clearly show healthy mitochondria (the organelle most likely to be observably impacted by fluorescence based imaging) beyond 48 hours.
Featured video: Preadipocyte cell.
Dapi exposition led to complete cell shrinkage and rounding, followed by extensive blebbing at all intensities with 100ms exposure time. At 10% intensity and 100ms exposure time, loss of cell adhesion and cell shrinkage took place in 8 minutes.
Imaging regimen: 30 minutes acquisition at a frequency of 1 image every 6 seconds.
To be eligible for the prize in addition to the time-lapse/movie we need:
- Name of microscope used
- Cost of Microscope
- Cost of fluorescence label
- Cell Type and experimental conditions (including frequency of imaging)
For any lab able to send us imaging over 48 hours long, showing mitochondria labelled via fluorescence and appearing healthy, we will send you a bottle of champagne and share your movie on social media to celebrate your success.
If no one is able to pass 48 hours we will send a bottle of champagne to the lab that provides the longest fluorescence based imaging of mitochondria before they show signs of damage.
As a comparison here is a label-free movie by Nanolive
Name of microscope: 3D Cell Explorer
Cost of Microscope: microscopy <30K Euro, incubator <10k Euro
Cost of fluorescence label: 0
Cell Type and experimental conditions (including frequency of imaging): Live imaging of mouse pre-adipocytes for 48 hours. 1 image per minute.