Marker-free visualization of fibrin network formation by MDA-MB-231 cells

The main event in blood coagulation is fibrin network formation as a result of the polymerization of the soluble plasma protein fibrinogen [1]. Alterations in this process lead to hemostatic and thrombotic disorders such as hemophilia [2] or thrombosis [3]. Additionally, fibrin deposition in tumor cells plays an important role in the formation of the tumor stroma and in angiogenesis [4]. Fibrin clots in cancer exist only within lesions, and their early identification represents a safe and effective method of diagnosis of invasive cancers [4]. The footage shows fibrin network formation in MDA-MB-231 cells, a cell line from human breast adenocarcinoma. An image was obtained every 20 seconds during 15 minutes on Nanolive’s 3D Cell Explorer.

[1] Palta, S., Saroa, R., & Palta, A. (2014). Overview of the coagulation system. Indian journal of anaesthesia58(5), 515–523. https://doi.org/10.4103/0019-5049.144643

[2] Brummel-Ziedins, K. E., Branda, R. F., Butenas, S., & Mann, K. G. (2009). Discordant fibrin formation in hemophilia. Journal of thrombosis and haemostasis : JTH7(5), 825–832. https://doi.org/10.1111/j.1538-7836.2009.03306.x

[3] Korte W, Poon M, -C, Iorio A, Makris M: Thrombosis in Inherited Fibrinogen Disorders. Transfus Med Hemother 2017;44:70-76. doi: 10.1159/000452864

[4] Obonai, T., Fuchigami, H., Furuya, F. et al. Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment. Sci Rep 6, 23613 (2016). https://doi.org/10.1038/srep23613

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6 new scientific publications using Nanolive cell imaging

We are delighted to announce SIX exciting new articles featuring Nanolive’s 3D Cell Explorer.  The articles come from users based in Taiwan, Poland, Spain, and Belgium, respectively. TP3, an antimicrobial peptide, inhibits infiltration and motility of glioblastoma...

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