Marker-free visualization of fibrin network formation by MDA-MB-231 cells

The main event in blood coagulation is fibrin network formation as a result of the polymerization of the soluble plasma protein fibrinogen [1]. Alterations in this process lead to hemostatic and thrombotic disorders such as hemophilia [2] or thrombosis [3]. Additionally, fibrin deposition in tumor cells plays an important role in the formation of the tumor stroma and in angiogenesis [4]. Fibrin clots in cancer exist only within lesions, and their early identification represents a safe and effective method of diagnosis of invasive cancers [4]. The footage shows fibrin network formation in MDA-MB-231 cells, a cell line from human breast adenocarcinoma. An image was obtained every 20 seconds during 15 minutes on Nanolive’s 3D Cell Explorer.

[1] Palta, S., Saroa, R., & Palta, A. (2014). Overview of the coagulation system. Indian journal of anaesthesia58(5), 515–523. https://doi.org/10.4103/0019-5049.144643

[2] Brummel-Ziedins, K. E., Branda, R. F., Butenas, S., & Mann, K. G. (2009). Discordant fibrin formation in hemophilia. Journal of thrombosis and haemostasis : JTH7(5), 825–832. https://doi.org/10.1111/j.1538-7836.2009.03306.x

[3] Korte W, Poon M, -C, Iorio A, Makris M: Thrombosis in Inherited Fibrinogen Disorders. Transfus Med Hemother 2017;44:70-76. doi: 10.1159/000452864

[4] Obonai, T., Fuchigami, H., Furuya, F. et al. Tumour imaging by the detection of fibrin clots in tumour stroma using an anti-fibrin Fab fragment. Sci Rep 6, 23613 (2016). https://doi.org/10.1038/srep23613

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