Discover how Nanolive’s LIVE Cytotoxicity Assay transforms cytotoxic drug development through high-resolution, label-free quantification of cell health and death. Our application note explores how this advanced technology enables real-time monitoring of cell death responses to various compounds, for the development of anti-cancer/cytotoxic therapeutics.
Enhance predictive value with Nanolive’s LIVE Cytotoxicity Assay
Discover how to:
- Use high-resolution, label-free imaging to track cell health, stress responses, and death kinetics
- Enhance the predictive value of cytotoxicity assays and improve the translational relevance of in vitro studies
- Reproduce the tumor microenvironment more accurately with co-culture experiments
- Identify therapeutic selectivity and off-target effects using only a single fluorophore
- Quantify apoptosis and necrosis without labels
Case Studies from the Cytotoxic Drug Development Application Note
Explore practical applications of Nanolive’s LIVE Cytotoxicity Assay through detailed case studies showcasing its effectiveness in detecting drug selectivity, screening cytotoxic drugs, and capturing drug resistance.
Case Study 1: Detecting selective and non-selective killing of cells in co-culture
Evaluate the selective cytotoxicity of different drugs on a co-culture of cancer and non-cancer cells. The assay quantifies the type and kinetics of cell death label-free, revealing selective and off-target effects crucial for biologically relevant drug development.
Case study 2: Drug screening in a 96-well plate
Investigate the phenotypic effects of multiple cytotoxic drugs in parallel, without the bias of fluorescent markers. The label-free assay’s sensitivity allows for precise characterization of cell death types and kinetics, demonstrating its potential for characterizing drug mechanism of action and quantifying dose-response.
Case Study 3: Capturing drug resistance
Examine the heterogeneous response of a cell culture to a cytotoxic drug. The assay identifies treatment-resistant subpopulations, providing insights into potential resistance mechanisms and informing better therapeutic strategies.
Nanolive’s LIVE Cytotoxicity Assay revolutionizes cytotoxic drug development by delivering high-content, real-time data for high biological relevance. Access our application note to explore how this advanced technology can enhance your research and drive innovation in drug discovery and development.
Transform your research with Nanolive’s innovative solutions. For more information or to request a demo, contact us here.
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