Nanolive’s 3D Cell Explorer enables you to perform accurate and quantitative 4D spatio-temporal monitoring of nano-sized vesicular structures moving along the cytoskeleton network in living cells.
It allows you to:
- Track cell migration over time
- Measure cell speed
- Monitor morphological changes in cells during migration
- Measure cell’s properties during migration (adhering surface, volume, thickness, …)
Cell migration is essential for the proper development and maintenance of multicellular organisms. Tissue formation during embryonic development, wound healing and immune responses all require the coordinated movement of cells. Errors during this process can have serious consequences, including vascular disease, tumor formation and metastasis. An understanding of the mechanism by which cells migrate may lead to the development of novel therapeutic strategies for the well-being of human kind.
The 3D Cell Explorer allows for real-time and non-invasive cell migration monitoring in 3D. With the 3D Cell Explorer you can measure chemotaxis related migratory responses, effects of cytoskeletal abnormalities on cell migration patterns, quantitative tracking of cells of interest during time, …
Mouse Fibroblastic Reticular Cell (FRC) migration
Fibroblastic Reticular Cells (FRCs) were grown to 40% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100).
The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 2 hrs 54 minutes, capturing images every 30 seconds.
Human Monocyte migration among Red Blood Cells (RBC)
A sample of human blood was taken from a donor, smeared on a coverslip and imaged every 30 seconds at room temperature.
Amoeba migration & Hunting
Amoeba cells from Prof. Pierre Cosson (Department of Cell Physiology and Metabolism, University of Geneva Medical School) and Dr. Samuel Lord from (Department of Cellular and Molecular Pharmacology, HHMI, University of California, San Francisco) were imaged every 1.7 seconds in HL5 medium.
Learn more about the 3D Cell Explorer
The 3D Cell Explorer acts now as a cell GPS. Follow your cells and monitor their speed in real-time!