Nanolive’s 3D Cell Explorer allows you to perform accurate and quantitative 4D spatio-temporal monitoring of nano-sized vesicular structures moving along the cytoskeleton network in living cells.
It allows you to:
- Monitor the three major processes of cell death: apoptosis, necrosis and autophagy
- Assess morphological changes associated with cell death without any stain or sample preparation
Cell death is the event of a cell ceasing to carry out its functions. Programmed cell deaths (PCD), such as apoptosis and autophagy, is a controlled process that confers advantages during an organism’s life cycle. They are involved in a variety of biological events, including morphogenesis, maintenance of tissue homeostasis, and elimination of harmful cells. Malfunctioning of PCD leads to various diseases in humans, including cancer and several degenerative diseases. Necrosis, in contrast, is caused by factors external to the cell or tissue, such as infection, toxins, or trauma, which result in the unregulated destruction of cell components.
Nanolive’s new technology, the 3D Cell Explorer, enables to observe those processes in 3D, in real-time and in a non-invasive manner.
Fibroblastic Reticular Cells (FRCs): 3D images of a living cell structure with its organelles dying (due to external stimuli)
Mouse melanoma cancer cells (B16) necrosis
Mouse melanoma cancer cells (B16, p23) grown in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 8h, capturing images every 1 minute.
Fibroblastic Reticular Cells (FRCs) grown to ˞80% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 3h 40 minutes, capturing images every 30 seconds.