On this page you will have the possibility to explore Cell-Bacteria interactions as we see it: live, in 3D and stain free! Enjoy and stay tuned: the list of results grows every week…
The 3D Cell Explorer allows for:
- Label-free 3D infection process monitoring
- 3D localization of bacteria inside living cells
- Detection of mammalian cell infections
- Volume measurements of intra-cellular parasites
- Visualization of microorganism internalization process
Not only do cells interact among themselves, but they interact with other types of micro-organisms. Most of the time these interactions are harmful to the host. Bacteria try to interact with cells in order to invade them. They bind to the cell’s structures (glycolipids, glycoproteins,..) and try to modify their cytoskeleton. In other cases, these interactions are good for the host, such is the case for the gut microbiota. In both cases, researchers are trying to examine these interactions in detail. Nanolive’s new technology, the 3D Cell Explorer, allows the visualization of these interactions at a high temporal and spatial resolution. On top of that, thanks to the software STEVE, neither the cells nor the bacteria are harmed by a chemical staining, as this staining is done digitally based on their specific Refractive Index values.
Section of bee gut
Rehydrated paraffin embedded bee gut sections (5 µm) was imaged with the 3D Cell Explorer. The final “panoramic view” of the whole tissue section (more than 400 micrometers) was obtained by stitching multiple acquisitions by using a free online software. Like human intestine, bee gut is colonized by bacteria population. The most of the gut microbial community resides in the ileum.
Live HeLa Cells infected with Chlamydia trachomatis
Living HeLa cells infected by Chlamydia trachomatis were imaged with the 3D Cell Explorer. The parasite inclusion bodies are displayed in green inside the cell cytoplasm.
Learn more about the 3D Cell Explorer
Check out how the 3D Cell Explorer can be used for the monitoring and diagnosis of microbial infections!