On this page you will have the possibility to analyse the SURFACE AREA in real-time and stain free! Enjoy and stay tuned: the list of results grows every week…
It allows you to:
- Evaluate in real-time the available growing surface for cell culture
- Obtain a measure of the cellular growing speed
- Estimate the surface of 3D hydrogel and matrix
The most routine processes in cell culturing, such as passaging (process of sub-culturing cells), induction of differentiation or formulation of any repeatable experimental protocol requires that culture dish surface area covered by cells be carefully controlled and documented. Nanolive’s technology produces a fast (immediate results), accurate and marker-free measure of surface area based on their specific refractive index (RI). By capturing images at the beginning and at regular time intervals during the real-time acquisition, this methodology provides an accurate way to monitor the cell movement in 4D , and to quantify their migration rate.
Monitoring of surface covered by cancer cells
Mouse skin cancer cells (B16, p30) were grown to 100% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 12 hours, capturing images every 2 minutes.
Learn more about the 3D Cell Explorer
The 3D Cell Explorer acts now as a cell GPS. Follow your cells and monitor their speed in real-time!