Single cell characterizationLong term labelfree imaging of cell organelles, cell cycle analysis, endocytosis & 3D cell cultures
The only label-free live cell imaging tool that lets you characterize multiple cell organelles in high resolution and with high sensitivity based on their refractive index.
PLOS Biology: researchers capture several biological phenomena with Nanolive imaging
Researchers from EPFL reveal cellular behaviour using Nanolive imaging that allows label-free observation of novel shape and dry mass dynamics of mammalian organelles, including spinning nuclei.
Cell cycle analysis
Observation and characterization of cell shape, cell volume and cell organelles
Nanolive imaging allows for non-invasive long-term live cell imaging at very high spatio-temporal resolution. This breakthrough leads to the observation and characterization of continuous processes such as pre-mitotic nuclear rotation, mitosis, apoptosis, necrosis, autophagy, and pyroptosis in a new, holistic fashion.
Endocytosis refers to the process of internalization of substances into the cell. There are two main types of endocytosis: phagocytosis and pinocytosis. While during phagocytosis, large particles and bacteria are engulfed, in pinocytosis fluid and molecules contained in it are brought into the cell. Endocytosis involves cytoskeletal and structural modifications. The 3D Cell Explorer allows visualizing of fine membrane deformations and posterior vesicle formation that occur in endocytic processes.
Marker-free Imaging of Cryopreserved Human M1 Macrophages
In this video – obtained with Nanolive’s 3D Cell Explorer – we present cryopreserved human M1 macrophages from PromoCell in cell culture. The 3D Cell Explorer allows to image these living macrophages in a novel, marker-free fashion. A special note goes to the visualization of membrane ruffling as waves arising at the leading edge of lamellipodia that move centripetally toward the main cell body. Macrophages were imaged for over 24h at a frequency of 1 image every 10 seconds.
Cell drinking: a closer look on macropinocytosis
In this video we can observe how the membrane extends forming arm-like claws that reconnect resulting in a macropinosome. Once at the cytoplasm, the macropinosome fuses with a lysosome.
3D Cell Cultures
Mesenchymal stem cell interacting with 3D hydrogel
This video shows mesenchymal stem cells grown in 3D PEG hydrogels of varying degrees of stiffness. Cells were imaged for 48 hours at an acquisition rate of one image every 30 seconds. A post-processing colour scale was applied to data in the z-axis, to add spatial information to our images. Blue indicates where the cell is closer to the surface of the gel, while pink indicates where it is further away.