Stem cells label-free characterization

Observe stem cells in 3D over long periods of time at high spatial and temporal resolution with the 3D Cell Explorer
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The only label-free live cell imaging tool that lets you observe stem cells in 3D, over long periods of time at high spatial and temporal resolution and capture dynamic events in unparalleled detail.

Stem cell differentiating into neuronal cells

This video shows different stages of the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.

Human MSCs were grown in low-serum cell growth media in 35 mm dishes pre-coated with fibronectin. Then, neurogenic cell differentiation media was added to the cells, and changes were recorded using Nanolive’s CX-A.

Read the related blog post here.

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Stem cell differentiating into neuronal cells

This video shows the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.

Cells were grown for approx. 2 days in complete mesenchymal stem cell neurogenic differentiation medium before the differentiation process portrayed in this movie could be observed.

The cells in this footage were imaged for 20 hours every 4,5 minutes using a 5x5 grid and Nanolive’s CX-A.

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3D long-term live imaging of stem cells

Mouse Embryonic Stem Cells were imaged for 15 hours; 1 image every 15s. The cells are visibly healthy because they continue to divide during image acquisition.

The cells were imaged with the 3D Cell Explorer.

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High resolution imaging of mitosis in stem cells

In this video we can observe mitosis taking place in a living sample of human mesenchymal stem cells in spectacular detail. Cells were kindly provided by Promocell GmbH.

Read our detailed blogpost here.

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High resolution imaging of mitosis in stem cells

In this video we can observe mitosis taking place in a living sample of human mesenchymal stem cells in spectacular detail. Cells were kindly provided by Promocell GmbH.

Read our detailed blogpost here.

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Imaging stress fibers in stem cells

Human MSC isolated from bone marrow and kindly provided by PromoCell GmbH, were grown in fibronectin, which modified their shape. The use of fibronectin as substrate influences the cytoskeletal structures of the growing cells, leading to the formation of stress fibers in this case. The high concentration of proteins in stress fibers leads to a detectable refractive index that allows us to visualize their structure using the 3D Cell Explorer.

Read our detailed blogpost here.

 

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Feature Application

Feature application: Stem Cells

In this Feature Application, we show how Nanolive’s technology can be used to: (1) observe MSCs in 3D over long periods of time at high spatial and temporal resolution; (2) capture mitotic division events in MSCs in unparalleled detail; (3) observe the dynamics of stress fiber formation and (4) visualize the morphological changes that occur during stem cell differentiation.

Download our Feature Application Note >

Webinar 1

Watch our Webinar:
Long-term 3D imaging and quantification of stem cell dynamics

 

Dr. Mathieu Frechin, Head of Quantitative Biology at Nanolive introduces you to our holotomographic microscopy and its implications in long-term imaging of stem cells. He outlines the advantages that Nanolive’s holotomographic technology has to study sensitive cell lines. In particular, the following topics are being covered:

  • Challenges in live cell imaging of stem cells
  • State of the art of label-free microscopy for stem cells
  • Highlights of our holotomographic approach on stem cell research presenting movies and interesting biological dynamics.
Please register here to view the webinar on-demand:

Webinar 2

Watch our webinar:
Characterization of Stem cell differentiation dynamics with label-free live cell imaging

 

In this webinar, Dr. Emma Gibbin, Communications Specialist at Nanolive will:

  • discuss the unique challenges that stem cells pose to live cell imaging show how Nanolive’s new microscope, the CX-A, can overcome these difficulties
  • present never-seen-before footage of mesenchymal stem cells undergoing neural differentiation
  • discuss the dramatic phenotypic and morphological changes observed and the opportunities such research could offer

 

Please register here to view the webinar on-demand:

Video library

Long Term Live Imaging of Mouse Embryonic Stem Cells for 34 hours!

Long Term Live Imaging of Mouse Embryonic Stem Cells for 48 hours!

Live FUCCI mESC imaged for over 48 hours

Human Mesenchymal Stem Cell interacting with 3D hydrogel

Mitosis CD34+ Hematopoietic stem cells

Mitosis in Mesenchymal Stem Cells

Label-free live cell imaging of Mesenchymal Stem Cells undergoing mitosis

15 hours continuous imaging mouse Embryonic Stem Cells

Stem cell differentiation

Human stem cells 65h post incubation with neurogenic differentiation medium

Human stem cells incubated with neurogenic differentiation medium

High resolution live footage of the formation and spontaneous release of platelets

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Stem cell differentiation into neuronal cells

hMSC incubated with neurogenic differentiation medium

hMSC incubated with neurogenic differentiation medium

Compare Nanolive's microscopes

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging             

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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