Sensitive cells (Stem cells and primary cells)
Some type of cells like stem cells or primary cells are sensitive to experiment induced stresses such as phototoxicity or photobleaching caused by fluorescence microscopy, the current method of choice for cell imaging. One of the biggest challenges in cell biology is long-term imaging of fine cellular dynamics of sensitive cells. The 3D Cell Explorer generates no phototoxicity as it injects in the sample ~100 times less energy (~0.2 nW/µm) than light sheet microscopes (~1nW/µm2) that are the reference in the matter. With a resolution of 195 nm (higher than standard confocal) it enables high resolution and high-frequency imaging even with sensitive material.
Unique dynamics of cellular organelles or highly dynamic and small cellular structures can be clearly analyzed, such as mitochondria, lipid droplets, lamellipodia, nucleus, nucleoli, etc.
With Nanolive’s live cell imaging tool it is possible to perform endless live imaging at the maximal acquisition speed (1 image per 1.7 seconds).
This certainly exceeds any live cell imaging capabilities of any other microscope and will revolutionize live cell imaging of sensitive cells.
Stem cells: Advancing stem cell research with the 3D Cell Explorer
Scientists can discover more about the behavior of stem cells with Nanolive’s 3D live cell microscope – the 3D Cell Explorer. This microscope offers the best contrast, resolution and versatility that a label-free microscopic approach can offer today, coupled to unmatched time resolution. Last but not least, it’s 3D.
Check a couple of videos here below and if you want to learn more have a look at our stunning material here: https://nanolive.ch/applications/overview/single-cell-cell-culture-analysis/sensitive-cells/stem-cell-research/.
Primary cells are cells taken directly from living tissue (e.g. biopsy material) and put in culture. These cells are more representative of the main functional component of the tissue from which they are derived in comparison to continuous (tumor or artificially immortalized) cell lines thus representing a more representative model to the in vivo state, however they are also more sensitive to manipulation. This is why the 3D Cell Explorer offers an excellent solution to image these cells for long period of time without affecting their physiology.