Neuroscience

Long term non-invasive live cell imaging to investigate cellular neuroscience

The non-invasiveness of Nanolive’s label- and phototoxicity-free technology means very sensitive cells such a neuronal cells can be imaged for long-term periods of time, without handling or experimentally-induced perturbations.

Human Mesenchymal Stem Cell Differentiation into Neurons

To our knowledge this is the first high resolution long term live cell time-lapse movie imaging the differentiation of umbilical cord matrix human mesenchymal stem cells into neurons.

Cells were grown for 13 days in complete mesenchymal stem cell neurogenic differentiation medium before the differentiation process portrayed in this movie could be observed.

The cells in this footage were imaged for 20 hours every 30 seconds using Nanolive’s 3D Cell Explorer.

Cells and reagents were kindly provided by PromoCell.

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Neural plasticity in live rat hippocampal neurons

In this video we can observe rat hippocampal neurons in culture.

The cells were at 7 DIV, when they already become “mature neurons”. In this short video we can clearly observe two distinctive phenomena characterizing the normal physiology of this beautiful cell type: vesicle migration and neuronal plasticity.

A special thanks for the beautiful sample goes to the lab of prof. Šuput’s group at the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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Neural plasticity in live rat hippocampal neurons

In this video we can observe rat hippocampal neurons in culture.

The cells were at 7 DIV, when they already become “mature neurons”. In this short video we can clearly observe two distinctive phenomena characterizing the normal physiology of this beautiful cell type: vesicle migration and neuronal plasticity.

A special thanks for the beautiful sample goes to the lab of prof. Šuput’s group at the Institute of Pathophysiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia.

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Mitochondrial trafficking in neurons

In this video, neuronal mitochondria were stained using a fluorescent dye and then imaged for 10 mins with the 3D Cell Explorer-fluo at an acquisition frequency of 1 image every 10 secs.
Mitochondria can be seen being trafficked along axons, dendrites and within the cell body. They are also present in dendritic growth cones (actin-supported extensions of developing neurites).

Sample courtesy of Vanessa Morais and Rita Soares, iMM institute Lisbon.

Read our detailed blogpost here.

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Neuronal Mitochondria Dynamics

In this spectacular video of live neuron cells we can observe the dynamics and behavior of living and completely unperturbed mitochondria moving inside axons, dendrites and around the nucleus.

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Neuronal Mitochondria Dynamics

In this spectacular video of live neuron cells we can observe the dynamics and behavior of living and completely unperturbed mitochondria moving inside axons, dendrites and around the nucleus.

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Video library

Rat hippocampal neurons in culture. The purple colored video is digitally stained and in 3D

Stem cell differentiating into neuronal cells

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