Changes in mitochondrial morphology are good indicators of cell health. Nanolive offers the only tool able to image these organelles, their finest dynamics and interactions with other organelles (e.g. lipid droplets) marker-free and for long periods of time enabling researchers to extract more in-depth information about mitochondrial and cell health.
Mitochondria dynamics: fusions, fissions and mitochondrial network
This movie serves to draw your attention to fine scale mitochondria dynamics. Dynamic details of fission-fusion events and mitochondrial network remodelling are highlighted.
This video shows live imaging of mouse pre-adipocyte cells. Images were acquired for 48 hours at a rate of 1 image per minute.
The cells were imaged with the 3D Cell Explorer.
Live imaging of LD growth in HeLa cells after Oleic Acid loading using HTM. Movie length, 3 h; acquisition frequency, 1 image · min-1.
Lipid droplets dynamics
Mitochondria and lipid droplets provide a clearly distinguishable RI signal in two and three dimensions. This allows our instrument to image them live and marker-free, in contrast to other techniques which require, mitochondrial or lipid chemical markers.
Feature application: Cell Metabolism
In this Feature Application, we show that: (1) holotomography can resolve the spatial and temporal dynamics of mitochondria, LDs and mitochondria-LD interactions in unperturbed conditions; (2) it is possible to induce mitochondrial dysfunction and then restore functioning by the addition of a specific drug and (3) it is possible to induce and then visualize the early stages of LD formation.
Watch our Webinar: Mitochondria and lipid droplets in the spotlight: Label-free imaging of cell metabolism
Dr. Mathieu Frechin, Head of Quantitative Biology at Nanolive introduces you to the advantages of our holotomographic microscope for imaging key organelles supporting cellular metabolism such as mitochondria and lipid droplets. Watch how – for the first time – it is possible to image mitochondria live and completely marker free.
In particular, the following topics are being covered:
- Challenges in live cell imaging of cellular organelles
- State of the art of label-free microscopy of lipid droplets
- Highlights of our holotomographic approach on organelles live imaging
- Tracking lipid droplets in 3D
Please register here to view the webinar on-demand:
Video of label-free live cell imaging of a Preadipocyte cell with Nanolive imaging
Why do mitochondria look bean shaped in electron microscopy?
Mitochondrial Dynamics: fission and fusion in pre-adipocyte cells
Mitochondrial Dynamics in neurons