Immunology

Long term non-invasive live cell imaging of the immune system with Nanolive

The only label-free live cell imaging tool that lets you observe the interactions of T cells and macrophages.

APC T cell interaction/activation

In this movie, we can witness the T Cell activation process totally marker-free.

APC cells (Macrophages and dendritic cells obtained after isolation and in vitro differentiation of bone marrow cells from C57BL/6 mice) were incubated with freshly isolated naïve T cells and imaged with Nanolive’s 3D Cell Explorer for 11 minutes at a frequency of 1 image every 10 seconds.

* APCs are special cells that present the antigens on their surface so that other immune cells can “see” and detect it.

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Incredible 4D live imaging of T cell nuclear dynamics

4D live imaging of T cell nuclear dynamics for 19 minutes (images were taken every 15s).
Of additional interest in this acquisition is the sharp identification of the T cells’ nucleus and its dynamics that even allows for the observation of multilobulated nuclei in some of the cells.

Information on Z axis (depth) was processed so that a color scale (a gradient of color ranging from blue to pink) was applied to it, providing a sense of spacial organization in that axis. Full cells or cell components closer to the dish surface were colored blue, while pink accounted for cells or cellular content positioned further from the dish surface.

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Incredible 4D live imaging of T cell nuclear dynamics

4D live imaging of T cell nuclear dynamics for 19 minutes (images were taken every 15s).
Of additional interest in this acquisition is the sharp identification of the T cells’ nucleus and its dynamics that even allows for the observation of multilobulated nuclei in some of the cells.

Information on Z axis (depth) was processed so that a color scale (a gradient of color ranging from blue to pink) was applied to it, providing a sense of spacial organization in that axis. Full cells or cell components closer to the dish surface were colored blue, while pink accounted for cells or cellular content positioned further from the dish surface.

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The Perfect Murder – Macrophage Cell Killed by T cells

In this movie, we can observe macrophages and T cells interacting. Naive T cells are being presented with antigens by the macrophages which “instruct” T cells on what type of cells to target (such as cancer cells) and kill. During this interaction, T cells can play a role in immune system homeostasis by killing the macrophage presenting the antigen.

Read our detailed blogpost here.

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Video library

T cell killing macrophage and macrophages eating macrophage

The perfect murder – Macrophage cell killed by T cells

Marker-free Imaging of Cryopreserved Human M1 Macrophages

Living Macrophages Marker-free imaged for 24 hours

Living Macrophages Marker-free imaged for 4 hours

Compare Nanolive's microscopes

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging             

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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