Host-pathogen interactions

Long term non-invasive live cell imaging of host-pathogen interactions at a cellular level

The non-invasiveness of Nanolive’s label- and phototoxicity-free technology enables the study of pathogens and their role in disease progression and can help the development of vaccines and treatments.

Virology: Virus-induced cytopathic effects

 

Virus infections have a physical signature (Refractive Index) which can be detected with Nanolive live cell imaging.

In this footage, obtained using Nanolive’s 3D Cell Explorer-fluo we observe the progression of GFP-labelled adenovirus in human bone osteosarcoma epithelial (U2OS) cells. Refractive images were taken every 15 s for 25 mins, and fluorescence images were taken every 5 mins using the FITC channel.

Read our interview to Prof. Clodagh O’Shea from the Salk Institute for Biological Studies (La Jolla, CA, USA) here.

Learn more in our virology section.

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Bacterial infections: Listeria

 

In this video we can observe mouse macrophages that have been infected with Listeria monocytogenes. It shows cell division and listeria propagation to daughter cells.

Read our detailed blogpost here.

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Protozoa: P. falciparum’s intra-erythrocytic cycle, an important immune escape strategy in Malaria

Internalization of the parasite in the host red blood cells entails not just avoiding macrophages detection, but also degrading huge amounts of haemoglobin.

This loss of haemoglobin is visible under the 3D Cell Explorer. During its life cycle, the parasite’s shape does not remain constant. Indeed, its life cycle goes through different stages as shown in this video.

Read our detailed blogpost here.

Request a quote or demo >

 

Protozoa: P. falciparum’s intra-erythrocytic cycle, an important immune escape strategy in Malaria

Internalization of the parasite in the host red blood cells entails not just avoiding macrophages detection, but also degrading huge amounts of haemoglobin.

This loss of haemoglobin is visible under the 3D Cell Explorer. During its life cycle, the parasite’s shape does not remain constant. Indeed, its life cycle goes through different stages as shown in this video.

Read our detailed blogpost here.

Request a quote or demo >

 

Apicomplexa: Toxoplasma Gondii

 

In this footage, we can distinguish T. gondii’s apical ring-shaped complex in tachyzoites from a Toxoplasma gondii infection in mouse fibroblasts (MEF1 T2). This structure plays a key role in host cell invasion processes.
The 3D render obtained solely from the sample’s refractive index, an inherent characteristic from it (thus, from a non-stained sample) is compared to a static image obtained from confocal laser-scanning microscopy.

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Video library

E.coli being engulfed through phagocytosis

Read our blogpost here.

 

E.coli being engulfed through phagocytosis

Read our blogpost here.

 

 

Pneumonia bacteria phagocyted by neutrophils.

Read our blogpsost here.

Human papillomavirus infection in HeLa cells.

Read our blogpsost here.

Compare Nanolive's microscopes

3D CELL EXPLORER

Budget-friendly, easy-to-use, compact solution for high quality non-invasive 4D live cell imaging             

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3D CELL EXPLORER-fluo

Multimodal Complete Solution: combine high quality non-invasive 4D live cell imaging with fluorescence

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CX-A

Automated live cell imaging: a unique walk-away solution for long-term live cell imaging of single cells and cell populations

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