In this application note we will present the time lapse imaging of mouse embryonic stem cells (mESCs) that have been genetically modified to express the fluorescence ubiquitination cell cycle indicator (FUCCI), a two-color (red and green) indicator that allows to monitor the cell cycle phases.
We will explain how to use the 3D Cell Explorer-fluo to record movies that require both fluorescence and 3D refractive index imaging and will propose a solution to analyze the resulting time lapse experiment.
mESCs-FUCCI time lapse imaging. Follow this link to see the full movie: https://www.nanolive.ch/fucci-mesc/
Find the full application note here.
