User Testimonials“You don’t have to kill them to see how they live.” Federico Faggin, inventor of the microprocessor
Institute of Photonics and Electronics Academy of Sciences, Czech Republic
Dr. Michal Cifra’s lab has recently purchased Nanolive’s 3D Cell Explorer-fluo microscope and published his first paper using it in the scientific Journal of Photochemistry and Photobiology B: Biology (see here).
“We want to understand how various electromagnetic fields affect the remodeling of the cytoskeleton in real-time with all associated physiological events and Nanolive imaging is an excellent commercial solution for this. We want to use our 3D Cell Explorer-fluo to monitor morphological changes in cells in real time.”
KTH Royal Institute of Technology, Stockholm, Sweden
Dr. Patrick Sandoz has been collaborating with Nanolive since 2013, first as a PhD student and then as a post-doc in Prof. van der Goot’s lab at EPFL (Lausanne, Switzerland). He played a very important role in testing Nanolive’s first microscope, the 3D Cell Explorer.
“One of the major advantages of Nanolive imaging is that we can observe contact points between cells, migration patterns and different morphologies, all in an unperturbed system.”
Centre for Cancer Research (CRCL) in Lyon, France
“We use our 3D Cell Explorer in all of our on-going projects because it allows us to easily look at the mitochondria without the artifacts of staining or phototoxicity.”
Gabriel is one of 500 researchers currently based at the Centre for Cancer Research (CRCL) in Lyon, France. He created his lab “Cancer Cell Death” in October 2016 and has “been having fun killing cancer cells” ever since. The focus of his
lab is apoptosis, the most well-studied form of programmed cell death. Apoptosis has two main effectors: mitochondria and caspases, which have been extensively studied because of the pivotal role they play in many diseases, mainly in cancer.
Joseph T. Rodgers
Assistant Professor, Department of Stem Cell Biology and Regenerative Medicine, The Keck School of Medicine, University of Southern California, USA
“The Nanolive 3D Cell Explorer is a powerful tool to measure morphologic and functional features of live stem cells.
Our laboratory uses primary mouse and human skeletal muscle stem cells (MuSCs), also known as satellite cells, to study the mechanisms that regulate the injury-induced transition of stem cells from quiescent state into the cell cycle. This transition requires many days to complete. MuSCs are a model that allows us to study this transition ex vivo, immediately following FACS-mediated purification from muscle tissue. We have used the Nanolive 3D Explorer to visualize this process in never before seen detail.
read full testimonialThe accompanying video is of primary MuSCs isolated from a juvenile mouse (5-week-old). The video begins two hours after FACS isolation, each frame is 5 minutes at a frame rate of 18 frames/sec. MuSCs undergo profound changes in size, shape, and function during activation. In the first 24 hours after activation, there is a ~600% increase in cell volume and ~1,000% increase in metabolic activity prior to entering the cell cycle. MuSCs from juvenile mice require 35-40 hours to complete cytokinesis following activation. The kinetics of MuSC activation slow dramatically with age. MuSCs from an adult mouse require twice the amount of time to complete activation as MuSCs from juvenile mice. MuSCs from old mice require 3-4 times longer.
We use the Nanolive 3D Cell Explorer to perform high-resolution analysis of the morphologic and cell biologic features of MuSC activation. These data are producing new insights into the cellular processes involved in activation and mechanisms that underlie age-associated defects in MuSC activation.”
Shukry J. Habib, PhD
Principal Investigator, Centre for Stem Cells and Regenerative Medicine King¹s College London, London, UK
“This is an excellent technology that allow us to visualise cellular compartments and their rapid dynamics live without the need for staining/ florescence. In combination with advances in segmentation, it will open the door for unprecedented quantitative biology at high temporal resolution.”
Yasmine Abouleila and Ahmed Ali, PhD students
Research Associates, RIKEN Research Institute, Osaka, Japan
“It is amazing to be able to directly visualize the cell in its natural media, without labeling and in 3D. This allowed us to monitor the cell in 3D while sampling part of the cytoplasm and analyzing it using mass spectrometry, thus achieving quantitation on a subcellular space, possibly the first in the world to do so.
read full testimonialIn our lab, we are mainly concerned with understanding biology on a single cell level in a quantitative manner. The 3D Cell Explorer allows us to directly visualize the cell in 3D without labeling and with no sample treatment.
Using this technology we were able to be the first in the world to quantitate a biological molecule in subcellular space. By imaging a cell in 3D in less than 1.5 seconds, then taking another 3D image after sampling part of the cell with our proprietary method. By comparing the difference of the 3D image, we could measure the volume sampled in different compartments of the cell such as cytoplasm.”
Oliver Nayler, PhD
Head, Cardiovascular & Fibrosis Biology, Actelion Pharmaceuticals Ltd., Allschwil, Switzerland
“Actelion researchers were among the first to explore the potential of the Nanolive 3D Cell Explorer in cell biological applications within the pharmaceutical industry. We were very happy that the system is so easy to set-up (plug-and-play) and we are still amazed by the beautiful images it generates.
read full testimonialWe mainly use the Cell Explorer to perform real time image acquisition and we follow compound activity in different cellular backgrounds. In a very short period of time, the 3D Cell Explorer has become very intensively used and we have found applications in several different disease areas – we would not want to be without this instrument.”
Alain Geloen, PhD
CNRS Research Director, member of CarMeN Laboratory, Lyon, France
” […] It is like a window opened on a new world.” […] “What you did is fantastic. Your microscope is a great achievement. […] You bring a new way to see and to analyze cells. […] You give us the opportunity to read nature with an other physical quantity: the density.
read full testimonialWhen I work with your microscope I keep correcting myself, this is not optic [what I see] it is density. In matter of regulations, density is more important than optic. That is why I say we must restudy all the cell biology, not looking at the optic but through the density. I am fully convinced that this is the beginning of a new era in biology. […]
Clemens Grassberger, PhD
Research Fellow, Harvard Medical School & Radiation Oncology, Massachusetts General Hospital, Boston, USA
“The 3D Cell Explorer enables us to study chromatin condensation and nanoparticle uptake in live cancer cells, which wouldn’t be possible with other methods.
We are extending this work now to different cancer cell lines to explain variations seen in response to therapy.”
Wojtek Chrzanowski, MSc, PhD, DSc
Senior Lecturer, Faculty of Pharmacy and the Australian Institute for Nanoscale Science and Technology at the University of Sydney, Australia
“It is absolutely fantastic! Its ease of operation, intuitive nature, compact size, rapid imaging and no need for stains make this a system I would certainly recommend and it would certainly support many different kinds of research. In fact, this system would be valuable for secondary students studying biology too.”
read full testimonial
Read the full story from Dr. Chrzanowski here!
David Grunwald, PhD
Assistant Professor, Biochemistry and Molecular Pharmacology
UMASS Medical School (USA)
“I wanted to take holograms of cells since my early undergrad, not just cells, but LIVING cells. Why holograms, well simply because in semi-transparent cells light should be able to do more than ‘just’ Phase Contrast or DIC. In principle, it should be able to directly report on the refractive index of sub-cellular components!
read full testimonialWell and that we could use to report on metabolism, physiology, reaction to environmental change, stress… just think about it!
After years of reading either the early literature from the 1880’s onward or screening through PhD thesis works of current days it happens in December 2014: Philadelphia, the last corner of the exhibition hall during the ASCB annual meeting I run into a small booth and cannot understand why none of my lab members, but only a few of the biophysicists at the meeting are gathering here: a small machine taking holographic images of cells! Not from one of the major companies, but a solid small device that boils it down to the essential.
Now, it does not (maybe yet) do fluorescence but the journey has begun! Watch the evolution of a technology that will change the way you think about how cells work …”
Research Technician, Dr. Bryan Lo Lab, Ottawa Hospital Research Institute, Canada
“We’re very excited to have the 3D Cell Explorer in Ottawa! The instrument was extremely easy to set up; we plugged it in and started taking pictures immediately. I’m impressed with the amazing image resolution and the short acquisition time.
read full testimonialWe’re getting high quality images of many different cell types and samples – from live human and mouse cell lines in a culture dish to fixed cells mounted on a slide. We’re looking forward to testing the limits of the microscope and starting to do live cell imaging.”
Manuel Fankhauser, PhD
Biologist at the EPFL, Lausanne, Switzerland
“As a biologist, looking at cell morphology is a key requirement to perform meaningful experiments. To look at a cell in 3D, both from inside and from outside, this would greatly enhance our ability to perform successful experiments.”
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