Tissue morphological analysis and 3D characterization

On this page you will have the chance to analyze TISSUE MORPHOLOGY as we see it: in 3D and stain free! Enjoy and stay tuned: the list of results grows every week…

The 3D Cell Explorer allows for:

  • Label-free, 3D tissue visualizations (thickness up to 20 micrometers)
  • 3D Localization of ROI inside the tissue slide, based on its specific RI

Tissue morphological analysis is usually done on a sick patient’s biopsy. Thanks to the visualization of the tissue slide, the trained pathologist is able to give a diagnosis on the health of the patient. The usual approach for analyzing such slides is chemical staining which gives contrast to the transparent structures. The most common method is the Hematoxylin and Eosin (H&E) staining. However, this procedure requires a lot of reagents and time. Nanolive’s technology bypasses these limitations thanks to the digital staining of the slide, and you can now avoid all this preparation. Based on the structures’ physical properties (Refractive Index), the 3D Cell Explorer is able to replace the chemical staining with a digital one.

Mouse skin section observation with the 3D Cell Explorer

Paraffin fixed Mouse skin sections of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Portal triad in mouse liver

Paraffin fixed mouse kidney section of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Tissue section of mouse myocardium

Paraffin fixed mouse myocardium section of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Tissue section of mouse intestine

Paraffin fixed mouse trachea section of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Tissue section of mouse pancreas  

Paraffin fixed mouse pancreas section of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Tissue section of mouse kidney       

Paraffin fixed mouse kidney section of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Tissue section of mouse trachea

Paraffin fixed mouse trachea section of 8 µm. They were then dried overnight at 37°C before being dewaxed and rehydrated. Then they were rehydrated again, cleared in xylene and mounted in a xylene based glue.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

Find out the story behind a great technology, product and company

Related topics

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Study your cells in detail as they grow inside a 3D hydrogel matrix!

Check out 3D Cell Explorer’s holographic tomography vs immunofluorescence with Zebrafish skeletal muscle.  

Find our latest publications here

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