Electron microscope

Electron microscope

Headquartered in Lausanne and active since 2013, Nanolive presents an innovative tomographic microscope, able to observe what is happening inside a cell with exceptional resolution. Leading manufacturer of tomographic microscopes, Nanolive keeps innovating to meet challenges facing the cell exploration. The peculiarity of Nanolive’s microscope lie in the fact that it allows analysis of living cells without damaging them, that is to say without the use of markers. Nanolive’s team developed a microscope that rely on a unique technology, to allow the visualization of the living cellin 3D, instantly and without any stain. With its product, Nanolive hopes to conquer science sectors, such as medicine, biology and pharmacology, as well the education.

One of the microscope types contributing to the development of cellular exploration is the creation of electron microscopes. Innovative technologies after optical microscopes, electron microscopes allow researchers to interact with living cells, through a technology including electromagnetic lenses and electron beams. They are used to observe more microscopic organic structures and microorganisms. If the human eye can distinguish two objects separated by 0.2 mm, optical microscope can see objects separated by about 200 nm. Today, electron microscopes allow seeing atoms.

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The different models of electron microscopes

There are several types of electron microscopes, classified according to their final use. Despite their diversity, they are constituted by a number of common components. These common components include a pumping system to ensure proper vacuum in microscope chamber, an electron optical column containing an electron source, electron lenses, one or more detection systems, a floor holder sample for introduction and basic movements of the specimen during observation. Electrical and electronic parts are not left out, as they ensure the functioning of microscope components, and results recording and processing. Depending on their use, electron microscopes are grouped into several categories. There are, for example, ones used in sample geometry. Others concern conventional electron microscopes. These microscopes consist either of a scanning system or a photon system. Based on these categories, they are divided into four large families. The first family is gathered in conventional transmission microscopy. This type of microscopy uses electron microscope that allows observing the internal morphology of a biological material after prior preparation. This type of microscope operates by producing a radiation source which emits electrons, and these electrons move in an environment in high vacuum. As the vacuum does not work in a wet environment, it is necessary to dehydrate the samples after having them chemically fixed.

The samples are then cut into thin-section resins to facilitate their observation. The second family relates reflecting electron microscopy, which operates like a transmission electron microscope using an electron beam on a surface. However, instead of using a transmission, or secondary electrons, the reflected electron beam is detected, scattered by elasticity process. The third and fourth families use scanning electron microscopes. The scanning microscopy is a method that allows obtaining images of a sample surface. This technique is characterized by a dimensional effect of the image.

The specificities of the electron microscope

Unlike optical microscope, the image is not formed by a lens. It is rather formed sequentially by scanning the sample surface with an electron beam. The scanning electron microscope provides images of the area in connection with the electron scattering mode by the sample. The number of secondary and backscattered electrons emitted varies, depending on the impact point of the electron beam on the surface. These electrons are detected in the detectors. An image is obtained in relation to the electric current intensity at each point of the surface, and sample topography is thus obtained.

The third family is well consolidated in the reflecting scanning microscopy. This type of microscopy uses an electron microscope which provides images through interaction between an electron beam and the sample material to be observed. More specifically, it consists of an electron beam that scans the surface of the sample to be analyzed. In response to this scanning, the sample re-emits some particles which, when analyzed by different detectors, are used to reconstruct a 3D image. The last family concerns the scanning transmission electron microscopy. This microscopy method uses a type of microscope of which the operating principle combines certain aspects of the scanning and the transmission electron microscope. An electron source focuses an electron beam passing through the sample. A system of magnetic lenses allows the beam to sweep the surface of the sample to be analyzed.

As an internationally leading microscope manufacturer, Nanolive offers an innovative solution for science and biological research, as well as for education and medicine. Nanolive team employs a reliable system to monitor the quality of its high-tech microscope. In addition, highly-skilled and well-trained software specialists assist you in your work, and ensure that you get the most from your investment. Nanolive’s microscope is monitored by Steve, highly efficient software which can read and analyze microscope images, store cell image data, and print 3D rendering images.

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