Nanoparticle internalization

On this page you have the chance to explore Nanoparticles inside living cells as we see it: in 3D and stain free! Enjoy and stay tuned: the list of results grows every week

The unique behavior and properties of nanoparticles in a biological milieu allows us to address fundamentals questions in biology. Their applications in the biological/medical field are vast: procedures of cell manipulation (cell detection and separation), delivery agents (drug and gene delivery), cellular therapy (tumor destruction via heating, known as hyperthermia), or phagokinetic studies (follow internalization processes) and many others. In the field of biology, researchers need an innovative tool capable of monitoring all these processes. Nanolive’s new technology, the 3D Cell Explorer, allows us to track, in three-dimensions, nanoparticles in Real-Time and in a non-invasive way.

It allows for:

  • Label-free, real-time monitoring of Nanoparticles (NP) uptaking process 
  • Monitoring of living cell morphological changes due to NP interaction
  • Kinetic and Dynamic studies of NPs uptaking  
  • Intra and Extra-cellular 3D localization of NP in living cell cultures 
  • Volume measurements of intra-cellular nanoparticles loading

HUVEC cells incubated with gold nanoparticles

HUVEC (Human Umbilical Vein Endothelial Cells) grown to ˞40% confluency in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100) were fixed with 1%PFA for 10-15 minutes and then washed with PBS. The cells were treated with 40nm BPEI gold nanoparticles

Sample courtesy of Dr. Parwathy Chandran and Dr. Nancy A. Monteiro-Riviere – Nanotechnology Innovation Center of Kansas State University

HeLa Cells treated with Iron oxide nanoparticles

HeLa cells (Human cervical cancer cell line) grown to ˞30-40% confluency in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100) were fixed with 1%PFA for 10-15 minutes and then washed with PBS. The cells were treated with 1mg/ml of Iron oxide nanoparticles (7-20nm).

Sample courtesy of Debora Bonvin, LTP, EPFL, Switzerland

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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