Multi-layers

On this page you will have the possibility to explore the multi-layered cell culture as we see it: in 3D and stain free! Enjoy and stay tuned: the list of results grows every week…

It allows for:

  • Real-time, label-free monitoring of cellular multi-layer construction
  • Estimation of multi-layer thickness, homogeneity and distribution.
  • Differential staining, based on specific RI, of cellular subpopulation in multi-layer

Contact inhibition is a regulatory mechanism that functions to keep fibroblast-like cells growing in a monolayer. If a cell has plenty of available substrate space, it moves freely and replicates rapidly. This process continues until the cells occupy the entire available substratum. After that, normal cells will stop migrating and replicating (100% confluent culture). Cancer cells, in contrast, are generally insensitive to such contact inhibition. They continue moving after contact with their neighbors, migrating over adjacent cells and generating multi-layered cultures. Nowadays, new drug development requires simple, in vitro models that resemble the in vivo situation. This way, we are able to decrease the use of experimentation on animals. These multilayered cultures could be used as relatively simple three-dimensional systems with which to study the effects of microenvironmental conditions on anticancer drug activity. 

Fibroblastic Reticular Cells (FRCs) Multi-layers

Mouse Fibroblastic Reticular Cells (FRCs) were grown over 100% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). 

Learn more about the 3D Cell Explorer

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Related topics

Do you know that Bacteria grow in multi-layer as well “Bacteria multi-layer blog post

Observe a pretty nice FRC mitosis on multi-layer colture “FRCs multi-layer mitosis blog post“?

How it all began: low confluent cell colture. ‘Low confluency application page

Just a step before the overpopulation, how the cells reach the 100% confluency “High confluency application page

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