Cell division analysis

Nanolive’s 3D Cell Explorer enables you to perform accurate and quantitative 4D spatio-temporal monitoring of nano-sized vesicular structures moving along the cytoskeleton network in living cells.

It allows for:

  • Label-free 3D cell cycle analysis
  • Cell cycle correlated morphological changes
  • Measurement of cell cycle duration time 
  • Cell cycle phase distribution in a cell population
  • Detection of cell cycle anomalies
  • Volume measurements of sub-cellular parts during the cell cycle

The cell cycle is a fundamental process for mammalian cell growth and development. It is composed of an ordered series of events which describes the intervals of cell growth, DNA replication, and mitosis preceding cell division. Errors in mitosis causing the incorrect replication of DNA can result in deadly consequences for the organism, depending on the amount and type of errors. Cancer is an example of a pathology caused by harmful mutations which are multiplied by mitosis.

The 3D Cell Explorer allows for real-time and non-invasive cell cycle monitoring. It detects the refractive indices, in 3D, of each and every cell organelle, to a precision higher than 0.001, and with unprecedented nanometer resolution (x,y= 200nm; z=500nm) and can monitor changes in cellular RI, shape and size during the cell cycle and mitosis (e.g. DNA condensation). 

Mouse Fibroblastic Reticular Cell (FRC) mitosis

Fibroblastic Reticular Cells (FRCs) were grown to 60% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 45 minutes, capturing images every 30 seconds.

Mouse Embrionic Stem Cell (ESC) mitosis

Mouse Embryonic Stem Cells (mESCs) grown to 60% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 5h 30 minutes, capturing images every minute.

Multi-layer of FRC cells and mitosis 

p53 Knockout Fibroblastic Reticular Cells (FRCs) were cultured to over 100% confluency, where the cells grew on top of one another, in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 3h 40 minutes, capturing images every 30 seconds.

Cell cycle anomalies detection: Multipolar mitosis

Fibroblastic Reticular Cells (FRCs) grown to 80% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 3h 40 minutes capturing images every 30 seconds.

Monitoring of DNA refractive index changes during mitosis 

The refractive index of the DNA changes during mitosis. The 3D Cell Explorer is able to discriminate different mitotic phases based on these chromatin refractive index changes.  

If you are interested in receiving the digital staining panel used in this video, please feel free to contact us!

Cell thickness monitoring during mitosis

In the chart above, a mitotic cell was analyzed starting from prophase. The cell became progressively rounder and thicker while detaching from the bottom of the dish during the first mitotic phases and reaching a peak of 17.5 µm during the transition between anaphase and telophase. Later on, after the genetic material is segregated and the final cytokinesis takes place, the cell reattaches to the bottom of the culture dish and its thickness decreases to 8 µm. A new interphase is starting.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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Related topics

Read our successful blog post about “First Mitosis in 3D and without stain @ Nanolive!!

Are you wondering if your cells are close to dividing? You can use the 3D Cell Explorer to see  which phase your cells are in!

Watch, in 3D, as a gene knockout leads to multi-polar asymmetric divisions!

Watch cellular reproduction not only with mammalian cells, but with  yeast too!

Find our latest publications here

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