Low confluency

On this page you will have the possibility to explore the Cell culture with low confluency as we see it: in 3D and stain free! Enjoy and keep tuned: the list of results grows every week…

It allows you:

  • Real-time, label-free monitoring of single cell seeding and growing

In cell culture biology, confluence is the term commonly used as an estimate of the number of adherent cells in a culture dish or a flask, referring to the proportion of the surface which is covered by cells. For example, 50% confluency means that roughly half of the surface is covered. A regular and precise monitoring of the cell confluence status is fundamental for well-defined experimental planning: the efficacy of many transfection protocols  and subculturing procedures are strictly dependent on the cell culture confluent status. 

The 3D Cell Explorer allows you to acquire 3D images of your cell cultures in real-time and in a non-invasive way.

 

Lung cancer cells: low confluency

Mouse skin melanoma cancer cells (B16, p35) were grown to 25% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). 

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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Related topics

Discover more about co-colture in our blog post “Amoeba and mouse cancer cells” 

Extend your cell culture over the 2D limit: 3D cell culture – HeLa cells embedded in alginate beads 

Observe a pretty nice FRC mitosis on multi-layer colture “FRCs multi-layer mitosis blog post“?

Just a step before the overpopulation, cells reach the 100% confluency “High confluency application page

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