Intracellular trafficking

Nanolive’s 3D Cell Explorer enables accurate and quantitative 4D spatio-temporal monitoring of nano-sized vesicular structures moving along the cytoskeleton network in living cells.

It allows you to:

  • Discriminate vesicles and other cellular structures based on their specific refractive index, allowing for marker-free studies of this fascinating phenomenon.
  • Monitor the distribution of intracellular vescicles over time
  • Localize, in 3D, their accumulation in regions of interest (ROI).

Intracellular vesicle quantification over time. The number of vesicles was counted at different time points: t0; t1h; t2h; t3h.

A eukaryotic cell can be compared to a big city with several districts, each specialized in a particular role. For these districts to work properly in a coordinated manner, a complex infrastructure network of cargo modules (vesicles) and highways (communication) is needed. Throughout our cells, materials are continuously transported via membrane‐bound tiny packages called vesicles. These vesicles are delivered to specific intracellular locations by attaching them to molecular motors which haul them along cytoskeleton components, such as microtubules or actin filaments. This process is called intracellular trafficking and is crucial for the normal functioning of cells. Any defects in its regulation may cause various diseases: metabolic failures, neurodegenerative diseases such as Alzheimer’s or Parkinson’s diseases, and many senile genetic disorders.

Intracellular vesicular trafficking in Mouse melonoma cancer cell (B16) 

Mouse skin melanoma cancer cells (B16, p35) were grown to 40% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 8 hours, capturing images every minute.

Check our blog post: Intracellular delivery service

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