“You really need to be able to look at living cells because life is animate — it’s what defines life”
Eric Betzig, Nobel Prize Winner for Chemistry, 2014
Different microscopes, for different needs
A holographic tomographic microscope for non-invasive live cell imaging and without the need for any labeling
A holotomographic fluorescence microscope with 3 fluorescence channels
Applications: A tool for discovery
ANALYZE YOUR TRUE CELLS: DON’T LABEL THEM
Mouse embryonic stem cells during mitosis. Cell metrics and DNA condensation can be monitored during the whole process.
INVESTIGATE CELL CYCLE EVENTS IN 3D & 4D
Mouselanoma cells (Me 260.LN) undergoing Apoptosis.
IDENTIFY YOUR CELLS BASED ON THEIR REFRACTIVE INDEX
Mouse melanoma cells (B16) incubated with dictyostelium amoebae cells. The ΔRI 0.015 between the two cell types allows for segmentation of each cell populations.
CORRELATE STRUCTURE & FUNCTION
Add structural information to your acquisition. Correlate changes at the protein level with morphological and behavioural changes in your cells (e.g. FUCCI cells).
“You don’t have to kill them to see how they live.”
Federico Faggin, inventor of the microprocessor
Oliver Nayler, PhD
Head, Cardiovascular & Fibrosis Biology, Actelion Pharmaceuticals Ltd., Allschwil, Switzerland
“[…] In a very short period of time, the 3D Cell Explorer has become very intensively used and we have found applications in several different disease areas – we would not want to be without this instrument. […]”
Yasmine Abouleila and Ahmed Ali, PhD students
Research Associates, RIKEN Research Institute, Osaka, Japan
“[…] Using this technology we were able to be the first in the world to quantitate a biological molecule in subcellular space. By imaging a cell in 3D in less than 1.5 seconds, then taking another 3D image after sampling part of the cell with our proprietary method. […]”
Alain Geloen, PhD
CNRS Research Director, member of CarMeN Laboratory, Lyon, France
” […] It is like a window opened on a new world.[…]What you did is fantastic. Your microscope is a great achievement.[…]You bring a new way to see and to analyze cells.[…]You give us the opportunity to read nature with an other physical quantity: the density.[…]”
Clemens Grassberger, PhD
Research Fellow, Harvard Medical School & Radiation Oncology, Massachusetts General Hospital, Boston, USA
”The 3D Cell Explorer enables us to study chromatin condensation and nanoparticle uptake in live cancer cells, which wouldn’t be possible with other methods. […]”