Fluorescence

On this page you will have the possibility to explore the difference between Fluorescence and Nanolive’s new technology! Enjoy and stay tuned: the list of results grows every week

The 3D Cell Explorer allows for:

  • Comparative fluorescence microscopy acquisitions  
  • Correlative studies between RI and fluorescence intensity 
  • Specific signal colocalization analysis 

Traditional microscopy is limited by the need of chemical fluorescent molecule reporters in order to visualize the different cell components. The user has to decide prior to the experiment which stains they may need for the sample preparation. The typical time for this procedure is 1-72 hours and just a few stains are possible on the same sample. On top of that, samples need to be either fixed, meaning that cells are dead, or genetically modified, which can alter the behavior of the cells. Nanolive’s technology measures the Refractive Index distribution within the cell. Therefore, the user can decide after the experiment which cell parts they wish to ‘digitally stain’ in a limitless amount of colours – saving a lot of time and money on reagents. Stain and re-stain the cell over and over again without damaging it.

Sequential staining 

On the left panel, the result of an immunostaining protocol and confocal microscopy acquisitions (TOTAL TIME > 4 HOURS). Human Fibroblastic Reticular Cells (FRCs) were fixed with PFA and incubated with primary and secondary antibodies in order to stain cytoskeletal proteins. After that, the nuclei were stained with DAPI. On the right there are the 3D results of Nanolive’s technology. The cell, with any pre-processing step, was imaged with the 3D Cell Explorer and digitally stained with our software STEVE (TOTAL TIME < 10 MINUTES).

Zebrafish skeletral muscle

The micrograph on the left panel in the figure below shows an immunofluorescent staining of a cryosection (10 micrometer) of an adult zebrafish skeletal muscle. The cytoplasm of the myofibers is rich in contractile proteins such as myosins. Slow-twitch myofibers are characterized by the presence of a particular isoform of myosin heavy chain, the myosin heavy chain 1 (green). Mitochondria (red) have been labeled with an antibody directed against Tom20, a protein specifically expressed in the mitochondrial outer membrane. Mitochondria are present in the sub-sarcolemmal compartment (red filaments) as well as between the myofibrils (little spots inside green regions). Nuclei are stained with Hoechst, showed here in blue. The images acquired with the 3D Cell Explorer are displayed on the central and right panels: the grey scale image is a map of the tissue based on the Refractive Index (RI) of its components. The main structures were identified and reconstructed in 3D through our fast, easy, inexpensive and non-invasive digital staining procedure.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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