Automated Live Cell Imaging: The CX-A

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A unique walk-away solution for long-term live cell imaging

  • Organelles
  • Single Cell
  • Cell Populations
  • Label-free & 3D
  • Unperturbed Imaging
  • No Phototoxicity
  • No Photobleaching
  • Multi-well Plate

Observe living cells

From Population to Organelles

Nanolive’s CX-A extends the exploration of living cells from single cells to cell populations without compromising on the highest precision and resolution proper to all Nanolive products. It enables scientists to investigate macro cellular dynamics like cell health, proliferation, movement and function as well as micro organelle dynamics and interactions e.g. mitochondrial network characterization.

Stitching of 3x3 FoVs

Cell population

Single FoV

Single cell

Zoom into FoV

Organelle ecosystem

*RI: label-free Refractive Index

(Multiplex)³

Live cell specs

The CX-A is designed to work with 96 well plates to multiply and parallelize experimental conditions, hence, bringing undoubtable significance to each experiment and delivering solid biological insights to researchers (1).

Furthermore, the system is equipped with multiple imaging modalities to correlate and compare physical and chemical information at each time-point (2).

Finally, 3D data sets of every single image at every single time-point are automatically acquired in real-time (3). A fully integrated solution adapted to the most advanced professional needs.

1

96 well plate overview

2

Multiple organelle detection at each acquisition

1

96 well plate overview

2

Multiple organelle detection at each acquisition

3

3D acquisition (depth: 30 μm)

Long-term live cell incubation

Walk-away with automation

Nanolive’s CX-A automates data acquisition, thus delivering new insights and understanding into biological processes. 

Nanolive’s completely redesigned intuitive user-interface enables first-time users to set-up experiments in just a few minutes and walk away, while the CX-A automatically collects the images. In addition to improving data significance, multiple imaging regimens can be programmed within the same plate allowing users to run different applications in parallel.  A real-time preview allows the user to navigate through the data at any time while offering an overview of the experimental protocol. The 3D dataset output can then be exported with just a few clicks to multiple data formats for analysis.

Unique imaging technique

Image cells from seconds to weeks

The CX-A can image living cells for a virtually limitless amount of time. Thanks to its unique harmless way of cell preparation and observation, hundreds of images can be collected each hour, transforming endpoint assays to continuous analysis, for days or weeks, while cells remain unperturbed in a physiologically controlled environment. Users can freely define the perfect imaging regimen for their cells without worrying about phototoxicity or photobleaching.

These unique properties of Nanolive’s CX-A system enable users to continuously monitor their precious, most-sensitive cells and to study their finest behaviors without missing anything.

Time 0

Time 5h 30

Time 6h 32

Time 10h

See what you have been missing

Technical Specifications
1,3 Illumination source Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2)
Epifluorescence: High speed switchable <100 μs, Lifetime >20’000 hours
1,3 Resolution 3D Holotomography (HT): x,y: 200 nm; z: 400 nm
Epifluorescence: x,y: ~ 400 nm (depending on channel)
1,3 Field-of-view (FoV) Single FoV* / 2x2 FoV / 3x3 FoV
*Single FoV: RI: 90x90x30 μm; Fluorescence: 90x90 μm
1,3 Channels HT: Up to 8 organelles simultaneously
Epifluorescence: DAPI + FitC  + TritC | FitC + TritC + Cy5 | DAPI + FitC + TritC/Cy5
1,3 Imaging modalities Automated: 3D HT | 3D HT + Epifluorescence | 4D HT time-lapse | 4D HT + Epifluorescence time-lapse
2 Sample holder Nanolive’s 96 well plates, designed for high precision imaging with optical quality glass bottom and lid
1,3 Autofocus High precision label-free autofocus for stable long-term observations in all imaging modalities
5 Incubator stage-top Tokaihit stage top incubator: CO2 concentration range: 5% - 20% (±0.1%); Humidity: ~ 95%; Sample temperature: 30-40°C (±0.3°C)
1,3 Camera USB 3.0 CMOS Sony IMX174 sensor / Quantum Efficiency (typical) 70 % (at 545 nm) / Dark Noise (typical) 6,6 e¯ / Dynamic Range (typical) 73,7 dB
1,3 Microscope Objective Dry objective / 60x magnification / NA 0.8
1-5 Weight ~30Kg

Webinar

Watch our Webinar: Automated Label-free Live Cell Imaging with the CX-A

 

Dr. Mathieu Frechin, Head of Quantitative Biology at Nanolive will introduce you the CX-A and its functionalities. He will outline the possibilities for parallelized experiments and image production capabilities.

In particular, the following topics will be covered:

  • Nanolive’s imaging technology
  • Presentation of the new hardware and software
  • Acquisition highlights showing data quality and new possibilities for label-free drug screening
Sign up to watch the webinar:

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