Chris rocks!

Helloooooo!!

Here I am again!

It has been a kind of super busy week with many events taking place:

– A very nice event @ EPFL where we had the chance to present and interact with other start ups from here (thanks to the SV Post-doc association!),

– a quite demanding (;-)) Nanonight

– and tooooons of meetings (as usual!)

But now, finally, it is Friday, again, and Chris is testing!

Last Friday, Chris (and I, just watching him) got some veeeery nice results that I’ll share here with you today with a short description of our day and how it could have been….

Consider that IT WAS FRIDAY and at 5PM an apéro was starting at the department ;-)))) and tell me: which procedure would you chose????

DAY I (Thursday, in both cases…):

– Seed around 5’000 cells in a fluordish 35mm

II DAY:

CASE A

IMMUNOSTAINING

– Wash the cells 3x

– Fix the cells with 2% PFA for 15 minutes

– 1 hour of blocking/perm buffer

– 1st incubation with primary antibody for 1 hour at room temperature

– Wash 3x with PBS + 0.3% TX-100 for 15 minutes

– 2nd incubation with the secondary antibody for 1 hour at room temperature

– Wash 3x with PBS + 0.3% TX-100 for 15 minutes

– Last incubation with DAPI (1:10’000) for 30 minutes to 1 hour at room temperature

– Last washing

– Image with with fluo microscope

TOTAL TIME: 4 to 4h30.

CASE B

WITH OUR 3D CELL EXPLORER

– Switch on the microscope (1.30 min)

– Take your fluor dish from the incubator (depends on where your incubator is…)

– Insert the dish in the incubator

– Switch on Steve and find your favourite cell

– Start Exploring

– In one second get the first 3D image. You can decide if to stop the acquisition or if to get a time lapse

– Digitally stain your cell as you like with STEVE!

TOTAL TIME: 5 to 10 min.

Before&AfterNanolive

I am not even mentioning the costs of the reagents that with our technology are not needed!!

Many new experiments are in our minds… Keep on following us and share our progress! Your support really matters to us!!

PS: In this case since the imaged cell is the same, the cell was fixed… This would have not be needed if we would have just used the Cell Explorer!!

 

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