Cell death

Nanolive’s 3D Cell Explorer allows you to perform accurate and quantitative 4D spatio-temporal monitoring of nano-sized vesicular structures moving along the cytoskeleton network in living cells.

It allows you to:

  • Monitor of the three major process of cell death: apoptosis, necrosis and autophagy stain-free
  • Assess morphological changes associated with cell death without any stain or sample preparation

Cell death is the event of a cell ceasing to carry out its functions. Programmed cell deaths (PCD), such as apoptosis and autophagy, is a controlled process that confers advantages during an organism’s life cycle. They are involved in a variety of biological events, including morphogenesis, maintenance of tissue homeostasis, and elimination of harmful cells. Malfunctioning of PCD leads to various diseases in humans, including cancer and several degenerative diseases. Necrosis, in contrast, is caused by factors external to the cell or tissue, such as infection, toxins, or trauma, which result in the unregulated destruction of cell components.
Nanolive’s new technology, the 3D Cell Explorer, allows us to observe those processes in 3D, in real-time and in a non-invasive manner. 

Mouse melanoma cancer cells (B16) necrosis 

Mouse melanoma cancer cells (B16, p23) grown in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 8h, capturing images every 1 minute.

FRC apoptosis

Fibroblastic Reticular Cells (FRCs) grown to ˞80% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 3h 40 minutes, capturing images every 30 seconds.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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Related topics

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After cell division… Apoptosis vs Necrosis “Have you ever wondered how cells die“?

Check out the effect of toxic substances on cells as seen with the 3D Cell Explorer here! 

Watch as we use the 3D Cell Explorer to monitor the death of a Colpidium!

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