Cell cycle analysis

“It is absolutely fantastic! Its ease of operation, intuitive nature, compact size, rapid imaging and no need for staining." Dr. Wojtek Chrzanowski, University of Sydney
CELL DIVISION ANALYSIS

Mouse fibrobrastic reticular cells (FRCs)

Unveil kinetics, dynamics and morphological changes during mitosis

The 3D Cell Explorer is able to discriminate different mitotic phases based on these chromatin RI values

  • Monitor changes in nuclear RI, shape and size during mitosis (e.g. DNA condensation, chromosomes alignment and segregation).
  • Monitor changes in cellular shape and thickness during cell division
  • Measure the processes of surface attachment/detachment
  • Analyze chromosomes 3D shape and segregation path: The RI of the DNA dramatically changes during mitosis. 
CELL DEATH ANALYSIS: APOPTOSIS

T685A human melanoma cancer cell

Monitor in real-time cellular morphological changes induced by apoptosis

Study how the cell’s & organelles’ morphology and their optical properties progressively change during apoptosis

  • Measure cell’s RI changes during cell shrinkage
  • Analyze Cell & Nuclear volume modification & shrinkage
  • Follow the cell membrane remodeling and cell blebbing
  • Monitor mitochondria modifications during apoptosis
CELL DEATH ANALYSIS: NECROSIS

Mouse fibrobrastic reticular cells (FRCs)

Follow necrosis in real-time, at every second

Less than 2-second time scale resolution time-lapse allows to monitor the typical morphological features of necrosis death:

  • Observe plasma membrane breach
  • Measure the dilatation of the nuclear membrane
  • Monitor cell swelling
  • Observe the dynamic of the cell’s explosion
CELL DEATH ANALYSIS: AUTOPHAGY

Human glioblastoma cells

Observe the full autophagy process in real-time: no bleaching, no photo-toxicity = unlimited imaging time

Long term imaging (days) gives the possibility to monitor the complete autophagy process

  • Follow the accumulation & localization of autophagosomes
  • Evaluate RI modification and cell shrinkage
  • Analyze cell death due to Autophagy