Cell cycle analysis

CELL DIVISION ANALYSIS

Mouse fibrobrastic reticular cells (FRCs)

Unveil kinetics, dynamics and morphological changes during mitosis

The 3D Cell Explorer is able to discriminate different mitotic phases based on these chromatin RI values

  • Monitor changes in nuclear RI, shape and size during mitosis (e.g. DNA condensation, chromosomes alignment and segregation).
  • Monitor changes in cellular shape and thickness during cell division
  • Measure the processes of surface attachment/detachment
  • Analyze chromosomes 3D shape and segregation path: The RI of the DNA dramatically changes during mitosis. 
CELL DEATH ANALYSIS: APOPTOSIS

T685A human melanoma cancer cell

Real-time monitoring of cellular morphological changes induced by apoptosis

The cell & organelles morphology and their optical properties progressively change during apoptosis

  • Cell RI monitoring (increase of cellular density) during cell shrinkage
  • Cell & Nuclear volume modification & shrinkage
  • Follow the cell membrane remodeling and cell blebbing
  • Monitor mitochondria modifications during apoptosis
CELL DEATH ANALYSIS: NECROSIS

Mouse fibrobrastic reticular cells (FRCs)

Do not miss anything: follow necrosis in real-time

Less than 2-second time scale resolution time-lapse allows to monitor the typical morphological features of necrosis death:

  • Plasma membrane rupture
  • Dilatation of the nuclear membrane
  • Cell swelling
  • Release of inner component
CELL DEATH ANALYSIS: AUTOPHAGY

Human glioblastoma cells

Forget about limitations: no bleaching, no photo-toxicity = unlimited imaging time

Long term imaging (days) gives the possibility to monitor the complete autophagy process

  • Follow the accumulation & localization of autophagosomes
  • RI monitoring and volume reduction evaluation
  • Analyze cell death due to Autophagy