Cell-Cell interaction

On this page you will have the possibility to explore Cell-Cell interactions as we see it: live, in 3D and stain free! Enjoy and stay tuned: the list of results grows every week…

The 3D Cell Explorer allows for:

  • Label-free 3D interaction monitoring
  • Morphological and quantitative tracking of cells of interest
  • Morphological assessment of cell-cell interaction

There are various types of cell-cell interactions. In complex systems such as organs and tissues, cells are not isolated. They need to communicate between each other in order to adapt and thus survive to their environment. This kind of interaction happens when cells are in physical contact with each other and communication is done via the membrane’s proteins. However, another type of interaction can happen through the excretion of a signal (autocrine, paracrine, or endocrine). In this case, molecules are taken up by cells and cause a specific reaction, depending on the stimulation. In both cases, the need for a high spatial and temporal resolution microscope is crucial in order to observe the interactions. Nanolive’s technology, the 3D Cell Explorer, allows us to explore those mechanisms in a non-invasive, 3D and live manner. On top of that, cells are not altered by fluorescence manipulation which could lead to th detection of false interactions, but instead the staining is done post-acquisition in a digital manner thanks to our software STEVE.

Living T-cell killing a living cancer cell

ID8-ova cells (ID8 murine ovarian tumor cell line transduced with ovalbumin) seeded (˞2×104 cells) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100) and grown to ˞20-30% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium). The cells were incubated with ˞5×105 T-cells. Cells were imaged using imaging buffer (PBS + 25mM Glucose + 10mM HEPES). The time-lapse imaging experiment where conducted at RT for 41 minutes, capturing images every second.

Living T-cell interacting with a living antigen presenting cell 

Fibroblast reticular cells seeded (˞2×104 cells) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100) and grown to ˞20-30% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium). The cells were previously treated to express antigen specific receptors for T-cells, and then incubated with ˞5×105 T-cells. Cells were imaged using imaging buffer (PBS + 25mM Glucose + 10mM HEPES). The time-lapse imaging experiment where conducted at RT for 45 minutes, capturing images every second.

Cancer cells and amoeba in co-culture

Mouse skin melanoma cancer cells (B16, p35) were grown to 40% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). They were incubated overnight with dictyostelium amoebae cells (WT1, p14) grown in HL-5 medium. The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 8 hours capturing images every minute.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

Find out the story behind a great technology, product and company

Related topics

Check out our blog post about Cancer and Amoeba cells.

Observe your cells in a multi-layer culture with the 3D Cell Explorer.

Follow your cells in motion. Monitor cell migration with the 3D Cell Explorer!

Keep track of your cells’ confluency easily!

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