Cell-Bacteria interaction

On this page you will have the possibility to explore Cell-Bacteria interactions as we see it: live, in 3D and stain free! Enjoy and stay tuned: the list of results grows every week…

The 3D Cell Explorer allows for:

  • Label-free 3D infection process monitoring
  • 3D localization of bacteria inside living cells  
  • Detection of mammalian cell infections 
  • Volume measurements of intra-cellular parasites 
  • Visualization of microorganism internalization process

Not only do cells interact among themselves, but they interact with other types of micro-organisms. Most of the time these interactions are harmful to the host. Bacteria try to interact with cells in order to invade them. They bind to the cell’s structures (glycolipids, glycoproteins,..) and try to modify their cytoskeleton. In other cases, these interactions are good for the host, such is the case for the gut microbiota. In both cases, researchers are trying to examine these interactions in detail. Nanolive’s new technology, the 3D Cell Explorer, allows the visualization of these interactions at a high temporal and spatial resolution. On top of that, thanks to the software STEVE, neither the cells nor the bacteria are harmed by a chemical staining, as this staining is done digitally based on their specific Refractive Index values.

Section of bee gut

Rehydrated paraffin embedded bee gut sections (5 µm) was imaged with the 3D Cell Explorer. The final “panoramic view” of the whole tissue section (more than 400 micrometers) was obtained by stitching multiple acquisitions by using a free online software. Like human intestine, bee gut is colonized by bacteria population. The most of the gut microbial community resides in the ileum.

 

Live HeLa Cells infected with Chlamydia trachomatis

Living HeLa cells infected by Chlamydia trachomatis were imaged with the 3D Cell Explorer. The parasite inclusion bodies are displayed in green inside the cell cytoplasm.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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Related topics

Use the 3D Cell Explorer to watch Amoeba on the hunt for bacteria.

The D-Stain can automatically segment your cells!

Examine the gut and its microbiota!  Check out our blog about a honey bee’s gut microbiota

Check out how the 3D Cell Explorer can be used for the monitoring and diagnosis of microbial infections!

Find our latest publications here