Area

On this page you will have the possibility to analyse the SURFACE AREA in real-time and stain free! Enjoy and stay tuned: the list of results grows every week…

 It allows you to:

  • Evaluate in real-time the available growing surface for cell culture
  • Obtain a measure of the cellular growing speed
  • Estimate the surface of 3D hydrogel and matrix

The most routine processes in cell culturing, such as passaging (process of sub-culturing cells), induction of differentiation or formulation of any repeatable experimental protocol requires that culture dish surface area covered by cells be carefully controlled and documented. Nanolive’s technology produces a fast (immediate results), accurate and marker-free measure of surface area based on their specific refractive index (RI). By capturing images at the beginning and at regular time intervals during the real-time acquisition, this methodology provides an accurate way to monitor the cell movement in 4D , and to quantify their migration rate.

Monitoring of surface covered by cancer cells

Mouse skin cancer cells (B16, p30) were grown to 100% confluency in complete DMEM medium (Dulbecco’s Modified Eagle Medium) in 35mm glass bottom culture dishes (FluoroDishes™ WPI, #FD35-100). The time-lapse imaging experiment was conducted with a standard top-stage incubator set to 37°C and 5% CO2 for 12 hours, capturing images every 2 minutes.

Learn more about the 3D Cell Explorer

Read more about the 3D Cell Explorer here and learn how to use it in 1 minute with this video

Discover all the secrets of the 3D Cell Explorer on our Introductory webinar

Learn how to digitally stain your cells with our software STEVE, in 1 minute

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Related topics

Check out our blog post all about monitoring cell confluency!

Read our successful blog post about “How to measure cell volume with high precision? Ask STEVE!

Follow your cells in motion. Monitor cell migration with the 3D Cell Explorer!

The 3D Cell Explorer acts now as a cell GPS. Follow your cells and monitor their speed in real-time!

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