The 3D Cell Explorer-fluo

The world’s first holo-tomographic fluorescent microscope

Combine the best of two worlds

The most powerful solution to explore living cells

The 3D Cell Explorer-fluo answers to the scientific need to combine the benefits from non-invasive cell tomography with a well-established and recognized method: multi-channel fluorescence microscopy. This opens completely new frontiers for research.

Fluorescence microscopy offers the advantage to selectively visualize specific cell compartments by staining them with various fluorescent probes, while novel holographic tomography gives access to the whole 3D RI distribution within the cell. This combination allows to measure, for the first time, specific cell compartments in a totally non-invasive fashion, for an unlimited amount of time and in 3D.

Researchers can now have access to  two powerful imaging modalities at the same time; they can compare and correlate resulting data and add new information to their experiments. 

2 Imaging Modalities

 

The 3D Cell Explorer-fluo is equipped with 2 imaging modalities

Holographic tomography

Refractive index (RI) measurement

Holographic tomography

DAPI: nuclear stain

Multi-channel fluorescence

Physical structural imaging

  • Refractive Index (RI) measurement of the whole cell in 3D
  • 4D continuous non-invasive cell observation (for days at every second)
  • Digital Stains: Post-experiment segmentation of cell organelles through RI markers

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Chemical fluorescence imaging

  • Specificity and sensitivity of antibody-conjugated probes and genetically-engineered fluorescent protein constructs
  • Functional measurement of cell organelles, proteins or drugs
  • Well established scientific method

3D digital tomography of nuclei (and nucleoli)

  • Transform 2D fluorescence microscopy into 3D cell tomography

  • Reduce imaging artefacts

  • Explore 10 markers in parallel

Transform 2D fluorescence microscopy into 3D cell tomography

 

Unveil new dimensions!

Localize organelles at different cell depths  (e.g. mitochondria)

Calibrate Digital Stain from fluorescence signal: extend 2D into 3D

3D Digital Stain calibrated on mitochondria (DsRed) at different depths

Z=0μm

Z=+4μm

Z=+8μm

Tomography of mitochondria

Time-lapse of DsRed Fluorescence (Mitochondria) + 3D Refractive index measurement

Reduce imaging artefacts, get more and reliable cell measurements

 

Eliminate errors!

2D Fluorescence Stain

3D Digital Stain calibrated on Plasma membrane chemical stain (Fyn-GFP)

Explore 10 markers in parallel

First microscope to allow imaging of up to 10 channels simultaneously!

 

Digital Stains calibrated for various organelles in mouse melanoma skin cancer cell (B16). 

Multiple 3D organelles tomography at every second

Co-localize fluorescence (3 channels) & Digital Stains (7 channels at single acquisition). Define Digital stain libraries calibrated on fluorescence markers

 Technical Features 3D Cell Explorer-ƒluo

 

 

Holographic tomography

Fluorescence imaging

Illumination source

Class 1 laser low power (λ=520 nm,
sample exposure 0.2 mW/mm2)
 High speed switchable <100 μs
Lifetime >20’000 hours each channel

Resolution

∧x,y: 200 nm; ∧z: 400 nm

∧x,y: ~ 400 nm

Field-of-view

90x90x30 μm

 100x100 μm

Channels

Up to 9 simultaneous

3: DAPI (blue), GFP (green), TRITC (red)

Imaging

 3D / 4D (time-lapse)  2D
Time resolution  0.6 fps 3D RI frame  5 fps each channel
Camera

CMOS sensor – 73 dB of dynamic range / Quantum efficiency of 70 % Readout noise: 6.6 e 

Dimensions  38x45x17 cm  21x23x10 cm
Weight  8 kg  4 kg

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