The first holo-tomographic
fluorescence microscope

Combine the best of two worlds

The most powerful solution to explore living cells

The 3D Cell Explorer-f  is equipped
with 2 imaging modalities

Holographic tomography

Refractive index (RI) measurement

Holographic tomography

DAPI: nuclear stain

Multi-channel fluorescence

Physical structural imaging

  • Refractive Index (RI) measurement of the whole cell in 3D
  • 4D continuous non-invasive cell observation (for days at every second)
  • Digital Stains: Post-experiment segmentation of cell organelles through RI markers

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Chemical fluorescence imaging

  • Specificity and sensitivity of antibody-conjugated probes and genetically-engineered fluorescent protein constructs
  • Functional measurement of cell organelles, proteins or drugs
  • Well established scientific method

3D digital tomography of nuclei (and nucleoli)


  • Transform 2D fluorescence microscopy into 3D cell tomography

  • Reduce imaging artefacts

  • Explore 10 markers in parallel


Transform 2D fluorescence microscopy into 3D cell tomography

Unveil new dimensions!

Localize organelles at different cell depths  (e.g. mitochondria)

Calibrate Digital Stain from fluorescence signal: extend 2D into 3D

3D Digital Stain calibrated on mitochondria (DsRed) at different depths

Z=0μm

Z=+4μm

Z=+8μm

Tomography of mitochondria

Time-lapse of DsRed Fluorescence (Mitochondria) + 3D Refractive index measurement


Reduce imaging artefacts, get more and reliable cell measurements

Eliminate errors!

2D Fluorescence Stain

3D Digital Stain calibrated on Plasma membrane chemical stain (Fyn-GFP)


Explore 10 markers in parallel

First microscope to allow imaging of up to 10 channels simultaneously!

 

Digital Stains calibrated for various organelles in mouse melanoma skin cancer cell (B16). 

Multiple 3D organelles tomography at every second

Co-localize fluorescence (3 channels) & Digital Stains (7 channels at single acquisition). Define Digital stain libraries calibrated on fluorescence markers


Technical Features 3D Cell Explorer-ƒ

 

 

Holographic tomography

Fluorescence imaging

Illumination source

Class 1 laser low power (λ=520 nm,
sample exposure 0.2 mW/mm2)
 High speed switchable <100 μs
Lifetime >20’000 hours each channel

Resolution

∧x,y: 200 nm; ∧z: 400 nm

∧x,y: ~ 400 nm

Field-of-view

90x90x30 μm

 100×100 μm

Channels

Up to 9 simultaneous

3: DAPI (blue), GFP (green), TRITC (red)

Imaging

 3D / 4D (time-lapse)  2D
Time resolution  0.6 fps 3D RI frame  5 fps each channel
Camera

CMOS sensor – 73 dB of dynamic range / Quantum efficiency of 70 % Readout noise: 6.6 e 

Dimensions  38x45x17 cm  21x23x10 cm
Weight  8 kg  4 kg

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