The 3D Cell Explorer DiscoveryThe most affordable tomographic microscope in the world
Looking inside life, differently…
The 3D Cell Explorer Discovery is a high speed, high resolution and non-invasive tomographic microscope that can look deep inside biological systems. This allows you to record stunning 3D images of entire cells and tissue slices in just seconds and with a higher resolution than any conventional microscope on the market. With the 3D Cell Explorer Discovery, researchers and students can directly experience what happens inside living cells in real time!
Non-invasive: no chemicals / no fluorescent markers
Fast: One tomographic 3D image at every second
Nanometric resolution: shorter than the wavelength of light
Quantitative: 3D measurement of cell organelles’ refractive index
With the 3D Cell Explorer Discovery you can…
Explore single cells and cell culture stain-free & instantly
Measure volumes and distances and monitor their reaction to stimuli without worrying about bleaching or phototoxicity.
Observe different types of cells
Discover different types of cells with incredible resolution and in 3D. Characterize their internal compartments based on their physical properties (i.e. morphology and refractive index).
Follow the action of life
Observe and analyze cell behaviour and cell-cell interaction without interfering with their natural physiology.
ANALYZE YOUR TRUE CELLS: DON’T LABEL THEM
Perform label-free non-invasive accurate and quantitative 3D morphological monitoring of living single cells and cell cultures at every second.
Melanoma cell front and side view.
IMAGE 3D CELL CULTURES
Perform label-free, real-time imaging of 3D cell cultures, without stains, in 2 seconds.
HUVEC (Human Umbilical Vein Endothelial Cells) incubated with Gold Nanoparticles.
IDENTIFY YOUR CELLS BASED ON THEIR REFRACTIVE INDEX
Nanolive’s proprietary digital stain allows the discrimination of different cell types in a co-culture based on their specific refractive index (RI) signature.
Mouse melanoma cells (B16) incubated with dictyostelium amoebae cells. The ΔRI 0.015 between the two cell types allows for segmentation of each cell populations. On the right panel three amoeba cells were zoomed to appreciate the resolution of the system (x,y= 180 nm; z= 400nm) and the lateral view.
UNDERSTAND CELL-CELL INTERACTIONS
Unveil the secrets of host pathogen interactions, phagocytosis, microorganism internalization & intracellular 3D localization at every second!
Dictyostelium amoeba cells phagocytosis of bacteria (E. coli). Four slices of the Z-stack tomography [Z resolution: 330nm]. Bacteria in the digestive vacuoles inside the amoeba cells are detectable.
PERFORM MARKER-FREE CYTOLOGY & HISTOLOGY
Perform marker-free analysis of tissues & cell samples saving precious time and reducing sample manipulations.
Mouse skin sections. The left panel shows a classical mouse skin slice stained with hematoxin & eosin. On the right panel the same kind of sample (5um thick slice) was fi xed with PFA and imaged with the 3D Cell Explorer Discovery.
The 3D Cell Explorer Discovery Highlights
3D tomographic cell visualization
The 3D Cell Explorer-Discovery delivers a full 3D cell reconstruction at every second. This allows you to record stunning 3D images of entire cells and tissue slices in just seconds and with nanometric resolution.
Quantitative Refractive Index data
The 3D Cell Explorer-Discovery detects changes to light as it propagates through the cell and measures the quantitative refractive index within the cell identifying each organelle.
No preparation, no staining
Our disruptive technology allows, for the first time, to explore instantly a living cell in 3D without any labeling or chemical markers. No fixation + No labeling = No alteration of cell’s normal physiology & No results falsification!
Segment your data through digital stain
Explore your data in 3D using our interactive digital stains based on the cell’s physical refractive index.
The 3D Cell Explorer is a tool for discovery and we are just at the beginning of exploring all the potential fields of application!
Technical Features 3D Cell Explorer Discovery
|Resolution|| Δx,y = 200nm;
Δz = 400nm
|Field of view||~80μm|
|Depth of field||~30μm|
|Tomography frame rate||0.5 fps 3D image rate with full self-adjustement|
|Objective||Air with 60x magnification|
|Illumination Source||Class 1 laser low power (λ=520 nm, sample exposure 0.2 mW/mm2)|
|Accessible sample stage||60 mm of free access to the sample stage for sample manipulation|